Mouse CXCL5/LIX/ENA-78/GCP 2 PicoKine® Quick ELISA Kit

Mouse CXCL5 / LIX / ENA-78 / GCP 2 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Cxcl5 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate) . Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78 (ENA-78) . It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on humanchromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 34 32 34 33 1.08 3.2% Sample 2 166 180 162 152 165 10.04 6% Sample 3 388 401 390 458 409 28.57 6.9% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 35 34 32 34 33 1.08 3.2% Sample 1 35 34 32 34 33 1.08 3.2% Sample 2 166 180 162 152 165 10.04 6% Sample 2 166 180 162 152 165 10.04 6% Sample 3 388 401 390 458 409 28.57 6.9% Sample 3 388 401 390 458 409 28.57 6.9%

Synonyms

C-X-C motif chemokine 5; Cytokine LIX; Small-inducible cytokine B5; GCP-2 (1-78) ; GCP-2 (9-78) ; Cxcl5; Scyb5

Gene Name

Cxcl5

Gene ID

20311

UniProt

P50228

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V45-A118

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, EDTA, citrate)

Detection Range

15.6 pg/mL - 1,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 388 36 150 360 Standard deviation 2.17 8.13 16.93 2.48 8.1 20.52 CV (%) 6.2% 4.9% 4.7% 6.9% 5.4% 5.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 388 36 150 360 Standard deviation 2.17 8.13 16.93 2.48 8.1 20.52 CV (%) 6.2% 4.9% 4.7% 6.9% 5.4% 5.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 166 388 36 150 360 Mean (pg/ml) 35 166 388 36 150 360 Standard deviation 2.17 8.13 16.93 2.48 8.1 20.52 Standard deviation 2.17 8.13 16.93 2.48 8.1 20.52 CV (%) 6.2% 4.9% 4.7% 6.9% 5.4% 5.7% CV (%) 6.2% 4.9% 4.7% 6.9% 5.4% 5.7%

Sensitivity

<10 pg/mL

Function

May participate in the recruitment of inflammatory cells by injured or infected tissue. GCP-2 (1-78) and, more potent, GCP- 2 (9-78) attract neutrophils and are involved in neutrophil activation. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

14235 MW

Specificity

Natural and recombinant mouse Cxcl5

Anticoagulant

Heparin, EDTA or citrate

Gene Name Synonym

C-X-C motif chemokine 5

Subcellular Location

Secreted.

Protein Name

C-X-C motif chemokine 5

Sample Data

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.263 0.439 0.800 1.200 1.524 1.826 2.017 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.263 0.439 0.800 1.200 1.524 1.826 2.017 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.005 0.263 0.439 0.800 1.200 1.524 1.826 2.017 O.D. 0.005 0.263 0.439 0.800 1.200 1.524 1.826 2.017

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.