Mouse P-Selectin / CD62P PicoKine® Quick ELISA Kit

Mouse P-Selectin / CD62P PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Selp in cell culture supernatants, serum and plasma (citrate) . Sensitivity: 5pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1 (PSGL-1) . The standard product used in this kit is recombinant mouse P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 42-709 amino acids sequence with the molecular mass of 99.1KDa. As a result of glycosylation, the molecular mass of 190-200KDa is revealed by SDS-PAGE.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 596 594 610 551 587 22.09 3.7% Sample 2 2016 2063 1888 1979 1986 64.18 3.2% Sample 3 7521 7765 7893 8387 7891 315.75 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 596 594 610 551 587 22.09 3.7% Sample 1 596 594 610 551 587 22.09 3.7% Sample 2 2016 2063 1888 1979 1986 64.18 3.2% Sample 2 2016 2063 1888 1979 1986 64.18 3.2% Sample 3 7521 7765 7893 8387 7891 315.75 4% Sample 3 7521 7765 7893 8387 7891 315.75 4%

Synonyms

P-selectin; CD62 antigen-like family member P; Granule membrane protein 140; GMP-140; Leukocyte-endothelial cell adhesion molecule 3; LECAM3; Platelet activation dependent granule-external membrane protein; PADGEM; CD62P; Selp; Grmp

Gene Name

Selp

Gene ID

20344

UniProt

Q01102

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: W42-A709

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (citrate)

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 596 2016 7521 640 1834 7467 Standard deviation 28.6 106.84 443.73 32 105% 500.28 CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 596 2016 7521 640 1834 7467 Standard deviation 28.6 106.84 443.73 32 105% 500.28 CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 596 2016 7521 640 1834 7467 Mean (pg/ml) 596 2016 7521 640 1834 7467 Standard deviation 28.6 106.84 443.73 32 105% 500.28 Standard deviation 28.6 106.84 443.73 32 105% 500.28 CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7% CV (%) 4.8% 5.3% 5.9% 5% 5.9% 6.7%

Sensitivity

<5 pg/mL

Function

Ca (2+) -dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with PSGL1. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

83099 MW

Specificity

Natural and recombinant Mouse Selp

Anticoagulant

Citrate

Gene Name Synonym

P-selectin

Subcellular Location

Membrane; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the selectin/LECAM family.

Protein Name

P-selectin

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.024 0.529 0.890 1.529 2.239 2.470 2.432 2.485 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.024 0.529 0.890 1.529 2.239 2.470 2.432 2.485 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.024 0.529 0.890 1.529 2.239 2.470 2.432 2.485 O.D. 0.024 0.529 0.890 1.529 2.239 2.470 2.432 2.485

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.