Human Angiostatin Kringle 1-3/PLG PicoKine® Quick ELISA Kit

Ang K1-3 is a single, non-glycosylated polypeptide chain containing 259 amino acids. It represents a proteolytic fragment of plasminogen containing the first three kringle structures. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. It displays increased inhibitory activity (EDa?...a?€ =70 nM) relative to kringles 1-4 (EDa?...a?€ = 135 nM) .

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 241 249 248 219 239 12.09 5% Sample 2 1987 1892 1804 1733 1854 95.22 5.1% Sample 3 5716 5780 5230 5301 5506 243.6 4.4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 241 249 248 219 239 12.09 5% Sample 1 241 249 248 219 239 12.09 5% Sample 2 1987 1892 1804 1733 1854 95.22 5.1% Sample 2 1987 1892 1804 1733 1854 95.22 5.1% Sample 3 5716 5780 5230 5301 5506 243.6 4.4% Sample 3 5716 5780 5230 5301 5506 243.6 4.4%

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 241 1987 5716 218 1908 5845 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 241 1987 5716 218 1908 5845 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 241 1987 5716 218 1908 5845 Mean (pg/ml) 241 1987 5716 218 1908 5845 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 Standard deviation 12.05 79.48 337.24 13.73 91.58 432.53 CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4% CV (%) 5% 4% 5.9% 4.8% 4.8% 7.4%

Plasmin dissolves the fibrin of blood clots and acts as a proteolytic factor in a variety of other processes including embryonic development, tissue remodeling, tumor invasion, and inflammation. In ovulation, weakens the walls of the Graafian follicle. It activates the urokinase-type plasminogen activator, collagenases and several complement zymogens, such as C1 and C5. Cleavage of fibronectin and laminin leads to cell detachment and apoptosis. Also cleaves fibrin, thrombospondin and von Willebrand factor. Its role in tissue remodeling and tumor invasion may be modulated by CSPG4. Binds to cells. .

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted . Locates to the cell surface where it is proteolytically cleaved to produce the active plasmin. Interaction with HRG tethers it to the cell surface.

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.053 0.127 0.201 0.426 0.968 1.538 1.868 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.053 0.127 0.201 0.426 0.968 1.538 1.868 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.024 0.053 0.127 0.201 0.426 0.968 1.538 1.868 O.D. 0.024 0.053 0.127 0.201 0.426 0.968 1.538 1.868

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.