Rat sE-Selectin PicoKine® Quick ELISA Kit

Rat sE-Selectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Sele in cell culture supernatants, serum and plasma (heparin, citrate) . Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils.1 An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions.2 E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM) .3 A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1.4

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 345 375 350 321 347 19.17 5.5% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 3 4428 3889 4055 4258 4157 203.63 4.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 345 375 350 321 347 19.17 5.5% Sample 1 345 375 350 321 347 19.17 5.5% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 2 1737 1912 2076 1952 1919 121.35 6.3% Sample 3 4428 3889 4055 4258 4157 203.63 4.8% Sample 3 4428 3889 4055 4258 4157 203.63 4.8%

Synonyms

E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; Sele; Elam-1

Gene Name

Sele

Gene ID

25544

UniProt

P98105

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: W22-P494

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum and plasma (heparin, citrate)

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 345 1737 4428 315 1621 4077 Mean (pg/ml) 345 1737 4428 315 1621 4077 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 Standard deviation 17.68 132.01 292.24 14.8 149.13 309.85 CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6% CV (%) 4.4% 7.6% 6.6% 4.7% 9.2% 7.6%

Sensitivity

<10 pg/mL

Function

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis (By similarity) . .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

60080 MW

Specificity

Natural and recombinant rat Sele

Anticoagulant

Heparin or citrate

Gene Name Synonym

E-selectin

Subcellular Location

Cell membrane ; Single-pass type I membrane protein.

Sequence Similarities

Belongs to the selectin/LECAM family.

Protein Name

E-selectin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.153 0.275 0.514 0.860 1.224 1.607 1.831 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.153 0.275 0.514 0.860 1.224 1.607 1.831 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.007 0.153 0.275 0.514 0.860 1.224 1.607 1.831 O.D. 0.007 0.153 0.275 0.514 0.860 1.224 1.607 1.831

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.