Mouse sE-Selectin PicoKine® Quick ELISA Kit

E-selectin, also called endothelial-leukocyte adhesion molecule-1 (ELAM-1), is a cell surface glycoprotein expressed by cytokine-activated endothelium that mediates the adhesion of blood neutrophils. An increased expression of E-selectin has been observed in the arterial endothelium interacting with lymphocytes and macrophages in human atherosclerotic lesions. E-selectin plays a critical role in mediating tissue-specific homing of T cells into skin, and of primitive hematopoietic progenitor cells (HPCs) into bone marrow (BM) . A structurally and functionally related group of genes, lymph node homing receptor (LHR), granule membrane protein 140 (GMP-140), and endothelial leukocyte adhesion molecule 1 (ELAM-1) are shown to constitute a gene cluster on mouse and human chromosome 1. The standard product used in this kit is recombinant mouse E-Selectin, excluding intercellular E-Selectin and transmembrane domain. It consists of totally 557 amino acids with the molecular mass of 85.8KDa.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 290 284 281 6.53 2.3% Sample 2 1580 1395 1401 1536 1478 81.52 5.5% Sample 3 4329 4374 4283 4168 4288 76.65 1.7% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 272 280 290 284 281 6.53 2.3% Sample 1 272 280 290 284 281 6.53 2.3% Sample 2 1580 1395 1401 1536 1478 81.52 5.5% Sample 2 1580 1395 1401 1536 1478 81.52 5.5% Sample 3 4329 4374 4283 4168 4288 76.65 1.7% Sample 3 4329 4374 4283 4168 4288 76.65 1.7%

E-selectin; CD62 antigen-like family member E; Endothelial leukocyte adhesion molecule 1; ELAM-1; Leukocyte-endothelial cell adhesion molecule 2; LECAM2; CD62E; Sele; Elam-1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1580 4329 277 1451 4721 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 272 1580 4329 277 1451 4721 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 272 1580 4329 277 1451 4721 Mean (pg/ml) 272 1580 4329 277 1451 4721 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 Standard deviation 11.96 105.86 186.14 16.62 108.82 250.21 CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3% CV (%) 4.4% 6.7% 4.3% 6% 7.5% 5.3%

Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with PSGL1/SELPLG. May have a role in capillary morphogenesis.

Description|Quantity 96-well plate|1 lyophilized Standard| Biotinylated detection antibody (50x) |100 ul Avidin-Biotin-Peroxidase Complex (30x) |400 μl Sample diluent|30 ml Antibody diluent|12 ml Avidin-Biotin-Peroxidase Diluent|12 ml Wash Buffer (25x) |20 ml Color Developing Reagent (TMB) |10 ml Stop Solution|10 ml Plate Sealers|4

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.013 0.041 0.080 0.201 0.368 0.751 1.474 2.156 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.013 0.041 0.080 0.201 0.368 0.751 1.474 2.156 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 Concentration (pg/ml) 0 156 312 625 125000 25000 5000 10000 O.D. 0.013 0.041 0.080 0.201 0.368 0.751 1.474 2.156 O.D. 0.013 0.041 0.080 0.201 0.368 0.751 1.474 2.156

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) . Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution.