Rat Adiponectin PicoKine® Quick ELISA Kit

Rat Adiponectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Rat Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 292 307 293 277 292 10.61 3.6% Sample 2 1705 1526 1807 1787 1706 110.86 6.4% Sample 3 4269 4351 4667 4673 4490 182.33 4% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 292 307 293 277 292 10.61 3.6% Sample 1 292 307 293 277 292 10.61 3.6% Sample 2 1705 1526 1807 1787 1706 110.86 6.4% Sample 2 1705 1526 1807 1787 1706 110.86 6.4% Sample 3 4269 4351 4667 4673 4490 182.33 4% Sample 3 4269 4351 4667 4673 4490 182.33 4%

Synonyms

30 kDa adipocyte complement-related protein ; Adiponectin, C1Q and collagen domain containing ; Protein Adipoq ; Adipoq ; Acdc ; rCG_36676

Gene Name

Adipoq

Gene ID

246253

UniProt

Q8K3R4

Reactivity

Rat

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I19-N244

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Applications

ELISA

Standard

10 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

156 pg/mL - 10,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 292 1705 4269 265 1626 4075 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 292 1705 4269 265 1626 4075 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 292 1705 4269 265 1626 4075 Mean (pg/ml) 292 1705 4269 265 1626 4075 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 Standard deviation 16.93 126.17 170.76 19.61 133.33 167.07 CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1% CV (%) 5.8% 7.4% 4% 7.4% 8.2% 4.1%

Sensitivity

<10 pg/mL

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

26410 MW

Specificity

Natural and recombinant Rat Adipoq

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Adiponectin, C1Q and collagen domain containing

Protein Name

Adiponectin

Sample Data

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.153 0.227 0.387 0.690 1.252 2.000 2.397 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.153 0.227 0.387 0.690 1.252 2.000 2.397 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.047 0.153 0.227 0.387 0.690 1.252 2.000 2.397 O.D. 0.047 0.153 0.227 0.387 0.690 1.252 2.000 2.397

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.