Mouse Adiponectin PicoKine® Quick ELISA Kit

Mouse Adiponectin PicoKine® Quick ELISA Kit (90 minutes, 96 Tests) . Quantitate Mouse Adipoq in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

Product Specifications

Background

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin.

Reproducibility

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 567 603 628 571 592 24.91 4.2% Sample 2 2994 2867 2845 2816 2880 67.97 2.3% Sample 3 7572 7510 8167 7413 7665 295.03 3.8% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 567 603 628 571 592 24.91 4.2% Sample 1 567 603 628 571 592 24.91 4.2% Sample 2 2994 2867 2845 2816 2880 67.97 2.3% Sample 2 2994 2867 2845 2816 2880 67.97 2.3% Sample 3 7572 7510 8167 7413 7665 295.03 3.8% Sample 3 7572 7510 8167 7413 7665 295.03 3.8%

Synonyms

Adiponectin;30 kDa adipocyte complement-related protein; Adipocyte complement-related 30 kDa protein; ACRP30; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adipoq; Acdc, Acrp30, Apm1

Gene Name

Adipoq

Gene ID

11450

UniProt

Q60994

Reactivity

Mouse

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Immunogen

Expression system for standard: NS0; Immunogen sequence: E18-N244

Capture Antibody

Polyclonal antibody from goat

Detection Antibody

Polyclonal antibody from goat

Tissue Specificity

Synthesized exclusively by adipocytes and secreted into plasma.

Applications

ELISA

Standard

20 ng/Tube

Sample Type

Cell culture supernatants, serum, plasma (heparin, EDTA) and urine

Detection Range

312 pg/mL - 20,000 pg/mL

Precision

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 567 2994 7572 512 3121 7518 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 567 2994 7572 512 3121 7518 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 567 2994 7572 512 3121 7518 Mean (pg/ml) 567 2994 7572 512 3121 7518 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 Standard deviation 37.98 179.64 318.02 37.75 240.31 330.79 CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4% CV (%) 6.7% 6% 4.2% 7.1% 7.7% 4.4%

Sensitivity

<10 pg/mL

Function

Important adipokine involved in the control of fat metabolism and insulin sensitivity, with anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW. .

Components

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Storage Conditions

Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)

Calculated Molecular Weight

26809 MW

Specificity

Natural and recombinant full length Mouse Adipoq

Anticoagulant

Heparin or EDTA

Gene Name Synonym

Adiponectin

Subcellular Location

Secreted.

Sequence Similarities

Contains 1 C1q domain.

Protein Name

Adiponectin

Sample Data

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.021 0.068 0.126 0.393 0.623 1.039 1.452 2.063 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.021 0.068 0.126 0.393 0.623 1.039 1.452 2.063 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.021 0.068 0.126 0.393 0.623 1.039 1.452 2.063 O.D. 0.021 0.068 0.126 0.393 0.623 1.039 1.452 2.063

Material Required But Not Provided

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.