Human Adiponectin PicoKine® Quick ELISA Kit

Adiponectin (ADPN) is a hormone secreted by adipocytes that regulates energy homeostasis and glucose and lipid metabolism. Adiponectin is a new member of the family of soluble defense collagens, in hematopoiesis and immune responses. It is an important negative regulator in hematopoiesis and immune systems and raise the possibility that it may be involved in ending inflammatory responses through its inhibitory functions. Adiponectin is mapped to 3q27 and can protect the organism from systemic inflammation by promoting the clearance of early apoptotic cells by macrophages through a receptor-dependent pathway involving calreticulin. The standard product used in this kit is the product of gene recombination, consisting of 226 (19-244) amino acids with the molecular mass of 36KDa after glycosylation.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3726 3810 3849 3865 3812 53.79 1.4% Sample 2 12058 12135 11622 10357 11543 712.13 6.1% Sample 3 49927 48196 52754 50855 50433 1645.09 3.2% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 3726 3810 3849 3865 3812 53.79 1.4% Sample 1 3726 3810 3849 3865 3812 53.79 1.4% Sample 2 12058 12135 11622 10357 11543 712.13 6.1% Sample 2 12058 12135 11622 10357 11543 712.13 6.1% Sample 3 49927 48196 52754 50855 50433 1645.09 3.2% Sample 3 49927 48196 52754 50855 50433 1645.09 3.2%

Adiponectin ; Adiponectin, C1Q and collagen domain containing ; cDNA FLJ78108, highly similar to Homo sapiens adiponectin, C1Q and collagen domain containing (ADIPOQ), mRNA ; ADIPOQ ; hCG_1784052

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 2578.68 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 2578.68 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Mean (pg/ml) 3726 12058 49927 3646 11478 49590 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 2578.68 Standard deviation 182.57 940.52 2346.56 236.99 1033.02 2578.68 CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2% CV (%) 4.9% 7.8% 4.7% 6.5% 9% 5.2%

Description|Quantity|Volume Anti-tag Pre-coated 96-well Strip Microplate|1|12 strips of 8 wells Standard||2 Antibody A|1|3ml Antibody B|1|3ml Sample Diluent|1|15ml TBS-T Wash Buffer (25x) |1|12ml Color Developing Reagent (TMB) |1|10ml Stop Solution|1|10ml Adhesive Plate Sealers|2|Piece

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.038 0.141 0.241 0.433 0.820 1.245 1.539 2.037 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.038 0.141 0.241 0.433 0.820 1.245 1.539 2.037 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.038 0.141 0.241 0.433 0.820 1.245 1.539 2.037 O.D. 0.038 0.141 0.241 0.433 0.820 1.245 1.539 2.037

Microplate Reader capable of reading absorbance at 450nm. Automated plate washer (optional) Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500ml graduated cylinders. Test tubes for dilution. Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.