Human Serpin A1/alpha 1-Antitrypsin EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Alpha-1-antitrypsin?or?α1-antitrypsin? (A1AT, ?A1A, or?AAT) is a?protein?belonging to the?serpin?superfamily. It is encoded in humans by the?SERPINA1?gene.?The?gene?is located on the long arm of the fourteenth?chromosome? (14q32.1) .The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.Like all?serine protease inhibitors, A1AT has a characteristic?secondary structure?of?beta sheets?and?alpha helices.?Mutations?in these areas can lead to non-functional proteins that can?polymerise?and accumulate in the?liver (infantile hepatic cirrhosis) .

Intracellular, Kinases, Metabolism, Metabolism Processes, Pathways and Processes, Protease Inhibitors, Proteolysis/Ubiquitin, Response To Hypoxia, Serum Proteins

Inhibitor of serine proteases. Its primary target is elastase, but it also has a moderate affinity for plasmin and thrombin. Irreversibly inhibits trypsin, chymotrypsin and plasminogen activator. The aberrant form inhibits insulin-induced NO synthesis in platelets, decreases coagulation time and has proteolytic activity against insulin and plasmin.

Catalog number Description Quantity Storage of opened/reconstituted material EZ1634-CA Mouse anti-human Serpin A1/alpha 1-Antitrypsin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1634-DA Biotinylated goat anti-human Serpin A1/alpha 1-Antitrypsin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1634-ST Lyophilized recombinant human Serpin A1/alpha 1-Antitrypsin standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1634-CA Mouse anti-human Serpin A1/alpha 1-Antitrypsin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1634-DA Biotinylated goat anti-human Serpin A1/alpha 1-Antitrypsin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1634-ST Lyophilized recombinant human Serpin A1/alpha 1-Antitrypsin standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1634-CA Mouse anti-human Serpin A1/alpha 1-Antitrypsin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1634-CA Mouse anti-human Serpin A1/alpha 1-Antitrypsin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1634-DA Biotinylated goat anti-human Serpin A1/alpha 1-Antitrypsin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ1634-DA Biotinylated goat anti-human Serpin A1/alpha 1-Antitrypsin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1634-ST Lyophilized recombinant human Serpin A1/alpha 1-Antitrypsin standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ1634-ST Lyophilized recombinant human Serpin A1/alpha 1-Antitrypsin standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.063 0.112 0.217 0.403 0.679 1.050 1.612 2.028 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.063 0.112 0.217 0.403 0.679 1.050 1.612 2.028 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 Concentration (ng/ml) 0 1.56 3.12 6.25 12.5 25 50 100 O.D. 0.063 0.112 0.217 0.403 0.679 1.050 1.612 2.028 O.D. 0.063 0.112 0.217 0.403 0.679 1.050 1.612 2.028

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)