Human ANGPTL4/Angiopoietin Like 4/ARP4 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Angiopoietin-related protein 4 (Angptl4) is a protein that in humans is encoded by the ANGPTL4 gene. This gene is a member of the angiopoietin/angiopoietin-like gene family and encodes a glycosylated, secreted protein with a fibrinogen C-terminal domain. This gene is induced under hypoxic conditions in endothelial cells and is the target of peroxisome proliferation activators. By radiation hybrid analysis, Angptl4 gene is mapped to 19p13.3. ANGPTL4 contributed to tumor growth and protected cells from anoikis, a form of programmed cell death induced when contact-dependent cells detach from the surrounding tissue matrix.

Angiopoietin-related protein 4; Angiopoietin-like protein 4; Hepatic fibrinogen/angiopoietin-related protein; HFARP; ANGPTL4; ARP4, HFARP, PGAR; PP1158, PSEC0166, UNQ171/PRO197

Angiogenesis, Cancer, Cancer Metabolism, Cardiovascular, Cholesterol Metabolism, Growth Factors, Hypoxia, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Lipids/Lipoproteins, Metabolic Signaling Pathway, Metabolic Signaling Pathways, Metabolism, Metabolism of Lipids and Lipoproteins, Metabolism Processes, Pathways and Processes, Response To Hypoxia

Protein with hypoxia-induced expression in endothelial cells. May act as a regulator of angiogenesis and modulate tumorigenesis. Inhibits proliferation, migration, and tubule formation of endothelial cells and reduces vascular leakage. May exert a protective function on endothelial cells through an endocrine action. It is ly involved in regulating glucose homeostasis, lipid metabolism, and insulin sensitivity. In response to hypoxia, the unprocessed form of the protein accumulates in the subendothelial extracellular matrix (ECM) . The matrix-associated and immobilized unprocessed form limits the formation of actin stress fibers and focal contacts in the adhering endothelial cells and inhibits their adhesion. It also decreases motility of endothelial cells and inhibits the sprouting and tube formation (By similarity) . .

Catalog number Description Quantity Storage of opened/reconstituted material EZ0960-CA Mouse anti-human ANGPTL4/Angiopoietin Like 4/ARP4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0960-DA Biotinylated goat anti-human ANGPTL4/Angiopoietin Like 4/ARP4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0960-ST Lyophilized recombinant human ANGPTL4/Angiopoietin Like 4/ARP4 standard 40 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0960-CA Mouse anti-human ANGPTL4/Angiopoietin Like 4/ARP4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0960-DA Biotinylated goat anti-human ANGPTL4/Angiopoietin Like 4/ARP4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0960-ST Lyophilized recombinant human ANGPTL4/Angiopoietin Like 4/ARP4 standard 40 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0960-CA Mouse anti-human ANGPTL4/Angiopoietin Like 4/ARP4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0960-CA Mouse anti-human ANGPTL4/Angiopoietin Like 4/ARP4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0960-DA Biotinylated goat anti-human ANGPTL4/Angiopoietin Like 4/ARP4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ0960-DA Biotinylated goat anti-human ANGPTL4/Angiopoietin Like 4/ARP4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0960-ST Lyophilized recombinant human ANGPTL4/Angiopoietin Like 4/ARP4 standard 40 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ0960-ST Lyophilized recombinant human ANGPTL4/Angiopoietin Like 4/ARP4 standard 40 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Secreted . Secreted, extracellular space, extracellular matrix . The unprocessed form interacts with the extracellular matrix. This may constitute a dynamic reservoir, a regulatory mechanism of the bioavailability of ANGPTL4 (By similarity) . .

Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.036 0.114 0.227 0.405 0.556 0.913 1.473 1.998 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.036 0.114 0.227 0.405 0.556 0.913 1.473 1.998 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 Concentration (pg/ml) 0 625 1250 2500 5000 10000 20000 40000 O.D. 0.036 0.114 0.227 0.405 0.556 0.913 1.473 1.998 O.D. 0.036 0.114 0.227 0.405 0.556 0.913 1.473 1.998

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)