Human NT-4/NTF4/Neurotrophin-4 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Neurotrophin-4 (NT-4), also known as neurotrophin-5 (NT-5), is a protein that in humans is encoded by the NTF4 gene. Human NT-4 as well as a human NT-4 pseudogene colocalize to chromosome 19 band q13.3. NT-4 is a member of a family of neurotrophic factors, the neurotrophins, that control survival and differentiation of vertebrate neurons (2-4) . NT-4 is a neurotrophic factor that signals predominantly through the TrkB receptor tyrosine kinase. NT4 plays a physiological role essential for hippocampus- and amygdala-dependent long-term memory and hippocampal long-lasting LTP and that NT4 may be useful in the therapy of acquired disorders of learning and memory.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0475-CA Mouse anti-human NT-4/NTF4/Neurotrophin-4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0475-DA Biotinylated goat anti-human NT-4/NTF4/Neurotrophin-4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0475-ST Lyophilized recombinant human NT-4/NTF4/Neurotrophin-4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0475-CA Mouse anti-human NT-4/NTF4/Neurotrophin-4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0475-DA Biotinylated goat anti-human NT-4/NTF4/Neurotrophin-4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0475-ST Lyophilized recombinant human NT-4/NTF4/Neurotrophin-4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0475-CA Mouse anti-human NT-4/NTF4/Neurotrophin-4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0475-CA Mouse anti-human NT-4/NTF4/Neurotrophin-4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0475-DA Biotinylated goat anti-human NT-4/NTF4/Neurotrophin-4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ0475-DA Biotinylated goat anti-human NT-4/NTF4/Neurotrophin-4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0475-ST Lyophilized recombinant human NT-4/NTF4/Neurotrophin-4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ0475-ST Lyophilized recombinant human NT-4/NTF4/Neurotrophin-4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097 O.D. 0.021 0.072 0.117 0.195 0.362 0.702 1.288 2.097

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)