Human CTRP9 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

The C1QTNF9 gene is conserved in chimpanzee, Rhesus monkey, and zebrafish. This gene is mapped to 13q12.12. 42 organisms have orthologs with human gene C1QTNF9.

Complement C1q and tumor necrosis factor-related protein 9A; Complement C1q and tumor necrosis factor-related protein 9; C1QTNF9; C1QTNF9A; UNQ6503; PRO21380

Catalog number Description Quantity Storage of opened/reconstituted material EZ1881-CA Mouse anti-human CTRP9 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1881-DA Biotinylated goat anti-human CTRP9 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1881-ST Lyophilized recombinant human CTRP9 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1881-CA Mouse anti-human CTRP9 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1881-DA Biotinylated goat anti-human CTRP9 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1881-ST Lyophilized recombinant human CTRP9 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1881-CA Mouse anti-human CTRP9 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1881-CA Mouse anti-human CTRP9 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1881-DA Biotinylated goat anti-human CTRP9 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ1881-DA Biotinylated goat anti-human CTRP9 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1881-ST Lyophilized recombinant human CTRP9 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ1881-ST Lyophilized recombinant human CTRP9 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.7 187.5 375 750 1500 3000 O.D. 0.007 0.026 0.069 0.162 0.354 0.799 1.518 2.021 Concentration (pg/ml) 0 46.9 93.7 187.5 375 750 1500 3000 O.D. 0.007 0.026 0.069 0.162 0.354 0.799 1.518 2.021 Concentration (pg/ml) 0 46.9 93.7 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.7 187.5 375 750 1500 3000 O.D. 0.007 0.026 0.069 0.162 0.354 0.799 1.518 2.021 O.D. 0.007 0.026 0.069 0.162 0.354 0.799 1.518 2.021

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)