Human Hemopexin EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Hemopexin (or haemopexin; HPX), also known as beta-1B-glycoprotein, is a protein that in humans is encoded by the HPX gene and belongs to hemopexin family of proteins. Hemopexin binds heme with the highest affinity of any known protein. Its function is scavenging the heme released or lost by the turnover of heme proteins such as hemoglobin and thus protects the body from the oxidative damage that free heme can cause. In addition, hemopexin releases its bound ligand for internalisation upon interacting with a specific receptor situated on the surface of liver cells. This function of hemopexin is to preserve the body's iron.

Cardiovascular, Cofactors, Vitamins/Minerals, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Metabolism, Pathways and Processes, Signal Transduction, Vitamins/Minerals

Catalog number Description Quantity Storage of opened/reconstituted material EZ1466-CA Goat anti-human Hemopexin polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1466-DA Biotinylated goat anti-human Hemopexin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1466-ST Lyophilized recombinant human Hemopexin standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1466-CA Goat anti-human Hemopexin polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1466-DA Biotinylated goat anti-human Hemopexin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1466-ST Lyophilized recombinant human Hemopexin standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1466-CA Goat anti-human Hemopexin polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1466-CA Goat anti-human Hemopexin polyclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1466-DA Biotinylated goat anti-human Hemopexin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ1466-DA Biotinylated goat anti-human Hemopexin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1466-ST Lyophilized recombinant human Hemopexin standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ1466-ST Lyophilized recombinant human Hemopexin standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.023 0.042 0.086 0.127 0.256 0.506 0.892 1.820 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.023 0.042 0.086 0.127 0.256 0.506 0.892 1.820 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 Concentration (ng/ml) 0 3.12 6.25 12.5 25 50 100 200 O.D. 0.023 0.042 0.086 0.127 0.256 0.506 0.892 1.820 O.D. 0.023 0.042 0.086 0.127 0.256 0.506 0.892 1.820

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)