Mouse OPG (TNFRSF11B) /Osteoprotegerin EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system.

Atherosclerosis, Cardiovascular, Cytokines, Cytoskeleton/ECM, Extracellular Matrix, Immunology, Innate Immunity, Metabolism, Obesity, Signal Transduction, Structures, TNF Superfamily, Vascular Inflammation

Acts as decoy receptor for TNFSF11/RANKL and thereby neutralizes its function in osteoclastogenesis. Inhibits the activation of osteoclasts and promotes osteoclast apoptosis in vitro. Bone homeostasis seems to depend on the local ratio between TNFSF11 and TNFRSF11B. May also play a role in preventing arterial calcification. May act as decoy receptor for TNFSF10/TRAIL and protect against apoptosis. TNFSF10/TRAIL binding blocks the inhibition of osteoclastogenesis. .

Catalog number Description Quantity Storage of opened/reconstituted material EZ0481-CA Rat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0481-DA Biotinylated goat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0481-ST Lyophilized recombinant mouse OPG (TNFRSF11B) /Osteoprotegerin standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0481-CA Rat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0481-DA Biotinylated goat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0481-ST Lyophilized recombinant mouse OPG (TNFRSF11B) /Osteoprotegerin standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0481-CA Rat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0481-CA Rat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0481-DA Biotinylated goat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ0481-DA Biotinylated goat anti-mouse OPG (TNFRSF11B) /Osteoprotegerin polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0481-ST Lyophilized recombinant mouse OPG (TNFRSF11B) /Osteoprotegerin standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ0481-ST Lyophilized recombinant mouse OPG (TNFRSF11B) /Osteoprotegerin standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.018 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.018 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.018 O.D. 0.000 0.082 0.173 0.294 0.522 0.976 1.585 2.018

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)