Human RBP4/Retinol Binding Protein 4 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that in humans is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.

Retinol-binding protein 4; Plasma retinol-binding protein; PRBP; RBP; Plasma retinol-binding protein (1-182) ; Plasma retinol-binding protein (1-181) ; Plasma retinol-binding protein (1-179) ; Plasma retinol-binding protein (1-176) ; RBP4; PRO2222

Atherosclerosis, Cancer, Cardiovascular, Cofactors, Vitamins/Minerals, Diabetes, Diabetes-associated, Heart Disease, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction, Vitamins/Minerals

Delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, this prevents its loss by filtration through the kidney glomeruli.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0831-CA Mouse anti-human RBP4/Retinol Binding Protein 4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0831-DA Biotinylated goat anti-human RBP4/Retinol Binding Protein 4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0831-ST Lyophilized recombinant human RBP4/Retinol Binding Protein 4 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0831-CA Mouse anti-human RBP4/Retinol Binding Protein 4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0831-DA Biotinylated goat anti-human RBP4/Retinol Binding Protein 4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0831-ST Lyophilized recombinant human RBP4/Retinol Binding Protein 4 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0831-CA Mouse anti-human RBP4/Retinol Binding Protein 4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0831-CA Mouse anti-human RBP4/Retinol Binding Protein 4 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0831-DA Biotinylated goat anti-human RBP4/Retinol Binding Protein 4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ0831-DA Biotinylated goat anti-human RBP4/Retinol Binding Protein 4 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ0831-ST Lyophilized recombinant human RBP4/Retinol Binding Protein 4 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ0831-ST Lyophilized recombinant human RBP4/Retinol Binding Protein 4 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.006 0.029 0.066 0.108 0.215 0.495 1.074 1.866 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.006 0.029 0.066 0.108 0.215 0.495 1.074 1.866 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.006 0.029 0.066 0.108 0.215 0.495 1.074 1.866 O.D. 0.006 0.029 0.066 0.108 0.215 0.495 1.074 1.866

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)