Human JAM-A/F11R/Junctional Adhesion Molecule 1 EZ-Set™ ELISA Kit (DIY Antibody Pairs)

Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.

Junctional adhesion molecule A; JAM-A; Junctional adhesion molecule 1; JAM-1; Platelet F11 receptor; Platelet adhesion molecule 1; PAM-1; CD321; F11R; JAM1, JCAM; UNQ264/PRO301

Seems to play a role in epithelial tight junction formation. Appears early in primordial forms of cell junctions and recruits PARD3. The association of the PARD6-PARD3 complex may prevent the interaction of PARD3 with JAM1, thereby preventing tight junction assembly (By similarity) . Plays a role in regulating monocyte transmigration involved in integrity of epithelial barrier. Involved in platelet activation. In case of orthoreovirus infection, serves as receptor for the virus. .

Catalog number Description Quantity Storage of opened/reconstituted material EZ1349-CA Mouse anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1349-RB-DA Biotinylated goat anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1349-RB-ST Lyophilized recombinant human JAM-A/F11R/Junctional Adhesion Molecule 1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1349-CA Mouse anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1349-RB-DA Biotinylated goat anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1349-RB-ST Lyophilized recombinant human JAM-A/F11R/Junctional Adhesion Molecule 1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1349-CA Mouse anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1349-CA Mouse anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 monoclonal antibody (Capture Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1349-RB-DA Biotinylated goat anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) EZ1349-RB-DA Biotinylated goat anti-human JAM-A/F11R/Junctional Adhesion Molecule 1 polyclonal antibody (Detection Antibody) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EZ1349-RB-ST Lyophilized recombinant human JAM-A/F11R/Junctional Adhesion Molecule 1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EZ1349-RB-ST Lyophilized recombinant human JAM-A/F11R/Junctional Adhesion Molecule 1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cell junction, tight junction . Cell membrane ; Single-pass type I membrane protein . Localized at tight junctions of both epithelial and endothelial cells.

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.011 0.122 0.187 0.314 0.615 0.923 1.435 1.858 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.011 0.122 0.187 0.314 0.615 0.923 1.435 1.858 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.011 0.122 0.187 0.314 0.615 0.923 1.435 1.858 O.D. 0.011 0.122 0.187 0.314 0.615 0.923 1.435 1.858

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)