Mouse IL-17F/Interleukin-17F ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Interleukin 17F, also called IL17F is involved in the regulation of normal versus aberrant T-cell responses. This gene is mapped to 6p12.2. The protein encoded by this gene is a cytokine that shares sequence similarity with IL17. This cytokine is expressed by activated T cells, and has been shown to stimulate the production of several other cytokines, including IL6, IL8, and CSF2/GM_CSF. This cytokine is also found to inhibit the angiogenesis of endothelial cells and induce endothelial cells to produce IL2, TGFB1/TGFB, and monocyte chemoattractant protein-1. It is suggested that targeting IL17 and IL17F or antagonizing IL17R might mitigate neutrophil-mediated inflammation in CF.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0796-CA Mouse anti- mouse Il17f monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0796-DA Biotinylated goat anti- mouse Il17f polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0796-ST Lyophilized recombinant mouse Il17f standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0796-CA Mouse anti- mouse Il17f monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0796-DA Biotinylated goat anti- mouse Il17f polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0796-ST Lyophilized recombinant mouse Il17f standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0796-CA Mouse anti- mouse Il17f monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0796-CA Mouse anti- mouse Il17f monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0796-DA Biotinylated goat anti- mouse Il17f polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) EZ0796-DA Biotinylated goat anti- mouse Il17f polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0796-ST Lyophilized recombinant mouse Il17f standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0796-ST Lyophilized recombinant mouse Il17f standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.25 62.5 125 250 500 1000 2000 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209 O.D. 0.003 0.067 0.14 0.282 0.585 1.12 1.76 2.209

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)