Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB) -regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0394-CA Mouse anti- mouse Il1b monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0394-DA Biotinylated goat anti- mouse Il1b polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0394-ST Lyophilized recombinant mouse Il1b standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0394-CA Mouse anti- mouse Il1b monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0394-DA Biotinylated goat anti- mouse Il1b polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0394-ST Lyophilized recombinant mouse Il1b standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0394-CA Mouse anti- mouse Il1b monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0394-CA Mouse anti- mouse Il1b monoclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0394-DA Biotinylated goat anti- mouse Il1b polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) EZ0394-DA Biotinylated goat anti- mouse Il1b polyclonal antibody (Detection Antibody) 500 μl, 0.15 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0394-ST Lyophilized recombinant mouse Il1b standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0394-ST Lyophilized recombinant mouse Il1b standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 Concentration (pg/ml) 0 12.5 25 50 100 200 400 800 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898 O.D. 0.030 0.065 0.123 0.212 0.336 0.609 1.031 1.898

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)