Human CCL14 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Chemokine (C-C motif) ligand 14 (CCL14) is a small cytokine belonging to the CC chemokine family. It is also commonly known as HCC-1. It is produced as a protein precursor that is processed to generate a mature active protein containing 74 amino acids that and is 46% identical in amino acid composition to CCL3 and CCL4. This chemokine is expressed in various tissues including spleen, bone marrow, liver, muscle, and gut. CCL14 activates monocytes, but does not induce their chemotaxis. In addition, HCC-1 enhanced the proliferation of CD34+ myeloid progenitor cells. It was as effective as MIP-1 alpha, but about 100-fold less potent. Human CCL14 is located on chromosome 17 within a cluster of other chemokines belonging to the CC family.

Catalog number Description Quantity Storage of opened/reconstituted material EZ1123-CA Rabbit anti- human CCL14 polyclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1123-DA Biotinylated goat anti- human CCL14 polyclonal antibody (Detection Antibody) 500 μl, 7.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1123-ST Lyophilized recombinant human CCL14 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1123-CA Rabbit anti- human CCL14 polyclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1123-DA Biotinylated goat anti- human CCL14 polyclonal antibody (Detection Antibody) 500 μl, 7.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1123-ST Lyophilized recombinant human CCL14 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1123-CA Rabbit anti- human CCL14 polyclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1123-CA Rabbit anti- human CCL14 polyclonal antibody (Capture Antibody) 500 μl, 4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1123-DA Biotinylated goat anti- human CCL14 polyclonal antibody (Detection Antibody) 500 μl, 7.5 μg/mL (recommended dilution 1:100) EZ1123-DA Biotinylated goat anti- human CCL14 polyclonal antibody (Detection Antibody) 500 μl, 7.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1123-ST Lyophilized recombinant human CCL14 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1123-ST Lyophilized recombinant human CCL14 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.007 0.069 0.161 0.359 0.670 1.288 1.955 2.234 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.007 0.069 0.161 0.359 0.670 1.288 1.955 2.234 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.007 0.069 0.161 0.359 0.670 1.288 1.955 2.234 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1,000 O.D. 0.007 0.069 0.161 0.359 0.670 1.288 1.955 2.234 O.D. 0.007 0.069 0.161 0.359 0.670 1.288 1.955 2.234

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)