Human CRP ELISA Kit EZ-SetTM (DIY Antibody Pairs)

C Reactive Protein (CRP) is a major acute phase reactant synthesized primarily in the liver hepatocytes. It is composed of 5 identical, 21,500-molecular weight subunits. CRP mediates activities associated with preimmune nonspecific host resistance. CRP shows the strongest association with cardiovascular events. It is detectable on the surface of about 4% of normal peripheral blood lymphocytes. Acute phase reactant CRP is produced in the liver.

C reactive protein pentraxin related; C-reactive protein; C-reactive protein (1-205) ; CRP; CRP_HUMAN; MGC149895; MGC88244; Pentraxin 1; Pentraxin 1 short; PTX 1; PTX1

Apoptosis, Atherosclerosis, Cardiovascular, Chromatin Binding Proteins, Complement, DNA/RNA Binding, Epigenetics and Nuclear Signaling, Immunology, Innate Immunity, Macrophage/Inflammation, Metabolism, Vascular Inflammation

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 75.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 75.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 593 1488 8142 565 1551 8792 Mean (pg/ml) 593 1488 8142 565 1551 8792 Standard deviation 36.17 62.49 643.21 38.42 75.99 861.61 Standard deviation 36.17 62.49 643.21 38.42 75.99 861.61 CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8% CV (%) 6.1% 4.2% 7.9% 6.8% 4.9% 9.8%

Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Catalog number Description Quantity Storage of opened/reconstituted material EZ1316-CA Rabbit anti- human CRP polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1316-DA Biotinylated goat anti- human CRP polyclonal antibody (Detection Antibody) 500 μl, 3.2 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1316-ST Lyophilized recombinant human CRP standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1316-CA Rabbit anti- human CRP polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1316-DA Biotinylated goat anti- human CRP polyclonal antibody (Detection Antibody) 500 μl, 3.2 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1316-ST Lyophilized recombinant human CRP standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1316-CA Rabbit anti- human CRP polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1316-CA Rabbit anti- human CRP polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1316-DA Biotinylated goat anti- human CRP polyclonal antibody (Detection Antibody) 500 μl, 3.2 μg/mL (recommended dilution 1:100) EZ1316-DA Biotinylated goat anti- human CRP polyclonal antibody (Detection Antibody) 500 μl, 3.2 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1316-ST Lyophilized recombinant human CRP standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1316-ST Lyophilized recombinant human CRP standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.005 0.046 0.1 0.174 0.448 0.83 1.374 1.959 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.005 0.046 0.1 0.174 0.448 0.83 1.374 1.959 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.005 0.046 0.1 0.174 0.448 0.83 1.374 1.959 O.D. 0.005 0.046 0.1 0.174 0.448 0.83 1.374 1.959

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)