Mouse Mimecan ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Osteoglycin (also called mimecan) is a protein that in humans is encoded by the OGN gene. This gene encodes a protein which induces ectopic bone formation in conjunction with transforming growth factor beta. This protein is a small proteoglycan which contains tandem leucine-rich repeats (LRR) . The level of expression of this gene has been correlated with enlarged hearts and more specifically left ventricular hypertrophy. Mimecan belongs to a family of small leucine-rich proteoglycans (SLRPs) . The mimecan gene was expressed at a moderate level in the mouse pituitary gland by Northern blot analysis. Expression of mimecan mRNA and protein is also observed in the human anterior pituitary gland. The OGN gene was mapped to chromosome 9q22.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 372 1477 206 405 1503 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 190 372 1477 206 405 1503 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 190 372 1477 206 405 1503 Mean (pg/ml) 190 372 1477 206 405 1503 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 Standard deviation 8.17 29.38 96 12.77 36.85 121.74 CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1% CV (%) 4.3% 7.9% 6.5% 6.2% 9.1% 8.1%

Catalog number Description Quantity Storage of opened/reconstituted material EZ1617-CA Rabbit anti- mouse Ogn polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1617-DA Biotinylated goat anti- mouse Ogn polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1617-ST Lyophilized recombinant mouse Ogn standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1617-CA Rabbit anti- mouse Ogn polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1617-DA Biotinylated goat anti- mouse Ogn polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1617-ST Lyophilized recombinant mouse Ogn standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1617-CA Rabbit anti- mouse Ogn polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1617-CA Rabbit anti- mouse Ogn polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1617-DA Biotinylated goat anti- mouse Ogn polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) EZ1617-DA Biotinylated goat anti- mouse Ogn polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1617-ST Lyophilized recombinant mouse Ogn standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1617-ST Lyophilized recombinant mouse Ogn standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.003 0.026 0.051 0.121 0.247 0.564 1.015 1.805 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.003 0.026 0.051 0.121 0.247 0.564 1.015 1.805 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.003 0.026 0.051 0.121 0.247 0.564 1.015 1.805 O.D. 0.003 0.026 0.051 0.121 0.247 0.564 1.015 1.805

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)