Human APOE ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 833 1078 1215 930 1014 23.42 2.3% Sample 2 4330 4376 3395 3797 3975 112.48 2.8% Sample 3 18188 16358 17821 13284 16413 1193.21 7.3% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 833 1078 1215 930 1014 23.42 2.3% Sample 1 833 1078 1215 930 1014 23.42 2.3% Sample 2 4330 4376 3395 3797 3975 112.48 2.8% Sample 2 4330 4376 3395 3797 3975 112.48 2.8% Sample 3 18188 16358 17821 13284 16413 1193.21 7.3% Sample 3 18188 16358 17821 13284 16413 1193.21 7.3%

Occurs in all lipoprotein fractions in plasma. It constitutes 10-20% of very low density lipoproteins (VLDL) and 1-2% of high density lipoproteins (HDL) . APOE is produced in most organs. Significant quantities are produced in liver, brain, spleen, lung, adrenal, ovary, kidney and muscle.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 833 4330 18188 1052 4071 18942 Standard deviation 63.72 326.48 1047.63 38.29 127.83 1407.39 CV (%) 7.7% 7.5% 5.8% 3.6% 3.1% 7.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 833 4330 18188 1052 4071 18942 Standard deviation 63.72 326.48 1047.63 38.29 127.83 1407.39 CV (%) 7.7% 7.5% 5.8% 3.6% 3.1% 7.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 833 4330 18188 1052 4071 18942 Mean (pg/ml) 833 4330 18188 1052 4071 18942 Standard deviation 63.72 326.48 1047.63 38.29 127.83 1407.39 Standard deviation 63.72 326.48 1047.63 38.29 127.83 1407.39 CV (%) 7.7% 7.5% 5.8% 3.6% 3.1% 7.4% CV (%) 7.7% 7.5% 5.8% 3.6% 3.1% 7.4%

Mediates the binding, internalization, and catabolism of lipoprotein particles. It can serve as a ligand for the LDL (apo B/E) receptor and for the specific apo-E receptor (chylomicron remnant) of hepatic tissues.

Catalog number Description Quantity Storage of opened/reconstituted material EZ1455-CA Rabbit anti- human APOE polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1455-DA Biotinylated goat anti- human APOE polyclonal antibody (Detection Antibody) 500 μl, 2 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1455-ST Lyophilized recombinant human APOE standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1455-CA Rabbit anti- human APOE polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1455-DA Biotinylated goat anti- human APOE polyclonal antibody (Detection Antibody) 500 μl, 2 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1455-ST Lyophilized recombinant human APOE standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1455-CA Rabbit anti- human APOE polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1455-CA Rabbit anti- human APOE polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1455-DA Biotinylated goat anti- human APOE polyclonal antibody (Detection Antibody) 500 μl, 2 μg/mL (recommended dilution 1:100) EZ1455-DA Biotinylated goat anti- human APOE polyclonal antibody (Detection Antibody) 500 μl, 2 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1455-ST Lyophilized recombinant human APOE standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1455-ST Lyophilized recombinant human APOE standard 200 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 Concentration (ng/ml) 0 0.625 1.25 2.5 5 10 20 40 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004 O.D. 0.01 0.073 0.138 0.243 0.484 0.907 1.475 2.004

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)