Rat Galectin-1 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-1 may act as an autocrine negative growth factor that regulates cell proliferation. Its expression in Hodgkin Lymphoma has also been shown to mediate immunosuppression of CD8+ T-cells. Additionally, Galectin-1 is thought to play a role in creating immune tolerance in pregnancy.

Galectin-1; Gal-1; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; RL 14.5; S-Lac lectin 1; Lgals1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 243 1504 5437 262 1512 5561 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 243 1504 5437 262 1512 5561 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 243 1504 5437 262 1512 5561 Mean (pg/ml) 243 1504 5437 262 1512 5561 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 Standard deviation 15.31 85.71 260.98 17.29 110.4 428.2 CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7% CV (%) 6.3% 5.7% 4.8% 6.6% 7.3% 7.7%

Lectin that binds beta-galactoside and a wide array of complex carbohydrates. Plays a role in regulating apoptosis, cell proliferation and cell differentiation. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Strong inducer of T-cell apoptosis.

Catalog number Description Quantity Storage of opened/reconstituted material EZ1589-CA Rabbit anti- rat Lgals1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1589-DA Biotinylated goat anti- rat Lgals1 polyclonal antibody (Detection Antibody) 500 μl, 3.3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1589-ST Lyophilized recombinant rat Lgals1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1589-CA Rabbit anti- rat Lgals1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1589-DA Biotinylated goat anti- rat Lgals1 polyclonal antibody (Detection Antibody) 500 μl, 3.3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1589-ST Lyophilized recombinant rat Lgals1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1589-CA Rabbit anti- rat Lgals1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1589-CA Rabbit anti- rat Lgals1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1589-DA Biotinylated goat anti- rat Lgals1 polyclonal antibody (Detection Antibody) 500 μl, 3.3 μg/mL (recommended dilution 1:100) EZ1589-DA Biotinylated goat anti- rat Lgals1 polyclonal antibody (Detection Antibody) 500 μl, 3.3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1589-ST Lyophilized recombinant rat Lgals1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1589-ST Lyophilized recombinant rat Lgals1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.274 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.274 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.274 O.D. 0.079 0.174 0.258 0.407 0.684 1.056 1.57 2.274

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)