Human TIMP-4 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Tissue inhibitor of metalloproteinases 4, also called Metalloproteinase inhibitor 4, is an enzyme that in humans is encoded by the TIMP4 gene. This gene belongs to the TIMP gene family. The proteins encoded by this gene family are inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. This gene is mapped to 3p25.2. The secreted, netrin domain-containing protein encoded by this gene is involved in regulation of platelet aggregation and recruitment and may play role in hormonal regulation and endometrial tissue remodeling.

Angiogenesis, Cancer, Cardiovascular, Cell Biology, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Protease Inhibitors, Proteolysis/Ubiquitin

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 589 2056 122 601 1963 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 123 589 2056 122 601 1963 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 123 589 2056 122 601 1963 Mean (pg/ml) 123 589 2056 122 601 1963 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 Standard deviation 9.47 38.87 125.41 10.98 48.08 155.07 CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9% CV (%) 7.7% 6.6% 6.1% 9% 8% 7.9%

Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7 and MMP-9.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0524-CA Rabbit anti- human TIMP4 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0524-DA Biotinylated goat anti- human TIMP4 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0524-ST Lyophilized recombinant human TIMP4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0524-CA Rabbit anti- human TIMP4 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0524-DA Biotinylated goat anti- human TIMP4 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0524-ST Lyophilized recombinant human TIMP4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0524-CA Rabbit anti- human TIMP4 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0524-CA Rabbit anti- human TIMP4 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0524-DA Biotinylated goat anti- human TIMP4 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) EZ0524-DA Biotinylated goat anti- human TIMP4 polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0524-ST Lyophilized recombinant human TIMP4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0524-ST Lyophilized recombinant human TIMP4 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.054 0.14 0.206 0.375 0.66 1.127 1.843 2.229 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.054 0.14 0.206 0.375 0.66 1.127 1.843 2.229 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 Concentration (pg/ml) 0 78 156 313 625 1250 2500 5000 O.D. 0.054 0.14 0.206 0.375 0.66 1.127 1.843 2.229 O.D. 0.054 0.14 0.206 0.375 0.66 1.127 1.843 2.229

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)