Human TREML1 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Trem-like transcript 1 protein is a protein that in humans is encoded by the TREML1 gene. This gene encodes a member of the triggering receptor expressed on myeloid cells-like (TREM) family. The encoded protein is a type 1 single Ig domain orphan receptor localized to the alpha-granule membranes of platelets. This protein is involved in platelet aggregation, inflammation, and cellular activation and has been linked to Gray platelet syndrome. Alternative splicing results in multiple transcript variants.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 213 407 34 200 394 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 35 213 407 34 200 394 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 35 213 407 34 200 394 Mean (pg/ml) 35 213 407 34 200 394 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 Standard deviation 1.89 16.82 24.01 2.07 15.8 30.33 CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7% CV (%) 5.4% 7.9% 5.9% 6.1% 7.9% 7.7%

Catalog number Description Quantity Storage of opened/reconstituted material EZ1521-CA Rabbit anti- human TREML1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1521-DA Biotinylated goat anti- human TREML1 polyclonal antibody (Detection Antibody) 500 μl, 1.25 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1521-ST Lyophilized recombinant human TREML1 standard 1 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1521-CA Rabbit anti- human TREML1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1521-DA Biotinylated goat anti- human TREML1 polyclonal antibody (Detection Antibody) 500 μl, 1.25 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1521-ST Lyophilized recombinant human TREML1 standard 1 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1521-CA Rabbit anti- human TREML1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1521-CA Rabbit anti- human TREML1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1521-DA Biotinylated goat anti- human TREML1 polyclonal antibody (Detection Antibody) 500 μl, 1.25 μg/mL (recommended dilution 1:100) EZ1521-DA Biotinylated goat anti- human TREML1 polyclonal antibody (Detection Antibody) 500 μl, 1.25 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1521-ST Lyophilized recombinant human TREML1 standard 1 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1521-ST Lyophilized recombinant human TREML1 standard 1 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.002 0.08 0.155 0.307 0.639 1.063 1.748 2.166 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.002 0.08 0.155 0.307 0.639 1.063 1.748 2.166 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.002 0.08 0.155 0.307 0.639 1.063 1.748 2.166 O.D. 0.002 0.08 0.155 0.307 0.639 1.063 1.748 2.166

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)