Rat Angiopoietin-1 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Angiopoietin 1, also called ANG1 is a type of angiopoietin and is encoded by the gene ANGPT1. Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. This gene was mapped to 8q23.1. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme and inhibits endothelial permeability. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 311 1285 4930 287 1289 5041 Mean (pg/ml) 311 1285 4930 287 1289 5041 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 Standard deviation 18.97 60.39 295.8 18.65 70.89 317.58 CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3% CV (%) 6.1% 4.7% 6% 6.5% 5.5% 6.3%

Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.

Catalog number Description Quantity Storage of opened/reconstituted material EZ1295-CA Rabbit anti- rat Angpt1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1295-DA Biotinylated goat anti- rat Angpt1 polyclonal antibody (Detection Antibody) 500 μl, 2.3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1295-ST Lyophilized recombinant rat Angpt1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1295-CA Rabbit anti- rat Angpt1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1295-DA Biotinylated goat anti- rat Angpt1 polyclonal antibody (Detection Antibody) 500 μl, 2.3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1295-ST Lyophilized recombinant rat Angpt1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1295-CA Rabbit anti- rat Angpt1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1295-CA Rabbit anti- rat Angpt1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1295-DA Biotinylated goat anti- rat Angpt1 polyclonal antibody (Detection Antibody) 500 μl, 2.3 μg/mL (recommended dilution 1:100) EZ1295-DA Biotinylated goat anti- rat Angpt1 polyclonal antibody (Detection Antibody) 500 μl, 2.3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1295-ST Lyophilized recombinant rat Angpt1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1295-ST Lyophilized recombinant rat Angpt1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.151 0.192 0.247 0.329 0.522 0.868 1.421 2.159 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.151 0.192 0.247 0.329 0.522 0.868 1.421 2.159 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 313 625 1250 2500 5000 10000 O.D. 0.151 0.192 0.247 0.329 0.522 0.868 1.421 2.159 O.D. 0.151 0.192 0.247 0.329 0.522 0.868 1.421 2.159

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)