Human PLAT/TPA ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Plasminogen activator, tissue, also called PLAT or TPA is a serine protease. This gene encodes tissue-type plasminogen activator, a secreted serine protease which converts the proenzyme plasminogen to plasmin, a fibrinolytic enzyme. Tissue-type plasminogen activator is synthesized as a single chain which is cleaved by plasmin to a two chain disulfide linked protein. This gene was mapped to 8p11.21. This enzyme plays a role in cell migration and tissue remodeling. Increased enzymatic activity causes hyperfibrinolysis, which manifests as excessive bleeding; decreased activity leads to hypofibrinolysis which can result in thrombosis or embolism.

Tissue-type plasminogen activator; t-PA; t-plasminogen activator; tPA; Alteplase; Reteplase; Tissue-type plasminogen activator chain A; Tissue-type plasminogen activator chain B; PLAT

Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 512 925 244 732 1216 Standard deviation 11.65 24.1 50 17.57 42.5 81.5 CV (%) 6.1% 4.7% 5.4% 7.2% 5.8% 6.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 191 512 925 244 732 1216 Standard deviation 11.65 24.1 50 17.57 42.5 81.5 CV (%) 6.1% 4.7% 5.4% 7.2% 5.8% 6.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 191 512 925 244 732 1216 Mean (pg/ml) 191 512 925 244 732 1216 Standard deviation 11.65 24.1 50 17.57 42.5 81.5 Standard deviation 11.65 24.1 50 17.57 42.5 81.5 CV (%) 6.1% 4.7% 5.4% 7.2% 5.8% 6.7% CV (%) 6.1% 4.7% 5.4% 7.2% 5.8% 6.7%

Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a role in facilitating neuronal migration.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0897-CA Rabbit anti- human PLAT polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0897-DA Biotinylated goat anti- human PLAT polyclonal antibody (Detection Antibody) 500 μl, 3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0897-ST Lyophilized recombinant human PLAT standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0897-CA Rabbit anti- human PLAT polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0897-DA Biotinylated goat anti- human PLAT polyclonal antibody (Detection Antibody) 500 μl, 3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0897-ST Lyophilized recombinant human PLAT standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0897-CA Rabbit anti- human PLAT polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0897-CA Rabbit anti- human PLAT polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0897-DA Biotinylated goat anti- human PLAT polyclonal antibody (Detection Antibody) 500 μl, 3 μg/mL (recommended dilution 1:100) EZ0897-DA Biotinylated goat anti- human PLAT polyclonal antibody (Detection Antibody) 500 μl, 3 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0897-ST Lyophilized recombinant human PLAT standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0897-ST Lyophilized recombinant human PLAT standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.106 0.193 0.337 0.614 1.066 1.751 2.326 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.106 0.193 0.337 0.614 1.066 1.751 2.326 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.029 0.106 0.193 0.337 0.614 1.066 1.751 2.326 O.D. 0.029 0.106 0.193 0.337 0.614 1.066 1.751 2.326

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)