Human Transthyretin/TTR ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Transthyretin (TTR) is also known as prealbumin. It is mapped to chromosome region 18q11.2-q12.1. This gene encodes a carrier protein responsible for the transport of thyroid hormones and retinol. The protein consists of a tetramer of identical subunits. Due to increased stability of the tetramer form of this encoded protein in mouse, compared to the human protein, this gene product has a reduced tendency to form amyloid fibrils. In humans, this protein binds beta-amyloid preventing its aggregation and providing a neuroprotective role in Alzheimer's disease.

Detected in serum and cerebrospinal fluid (at protein level) . Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.94 5.59 11.4 2.08 5.84 12.7 Standard deviation 0.091 0.313 0.73 0.114 0.362 0.914 CV (%) 4.7% 5.6% 6.4% 5.5% 6.2% 7.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.94 5.59 11.4 2.08 5.84 12.7 Standard deviation 0.091 0.313 0.73 0.114 0.362 0.914 CV (%) 4.7% 5.6% 6.4% 5.5% 6.2% 7.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1.94 5.59 11.4 2.08 5.84 12.7 Mean (pg/ml) 1.94 5.59 11.4 2.08 5.84 12.7 Standard deviation 0.091 0.313 0.73 0.114 0.362 0.914 Standard deviation 0.091 0.313 0.73 0.114 0.362 0.914 CV (%) 4.7% 5.6% 6.4% 5.5% 6.2% 7.2% CV (%) 4.7% 5.6% 6.4% 5.5% 6.2% 7.2%

Catalog number Description Quantity Storage of opened/reconstituted material EZ1684-CA Rabbit anti- human TTR polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1684-DA Biotinylated goat anti- human TTR polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1684-ST Lyophilized recombinant human TTR standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1684-CA Rabbit anti- human TTR polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1684-DA Biotinylated goat anti- human TTR polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1684-ST Lyophilized recombinant human TTR standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1684-CA Rabbit anti- human TTR polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1684-CA Rabbit anti- human TTR polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1684-DA Biotinylated goat anti- human TTR polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) EZ1684-DA Biotinylated goat anti- human TTR polyclonal antibody (Detection Antibody) 500 μl, 2.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1684-ST Lyophilized recombinant human TTR standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1684-ST Lyophilized recombinant human TTR standard 100 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.05 0.083 0.107 0.135 0.232 0.505 1.079 2.263 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.05 0.083 0.107 0.135 0.232 0.505 1.079 2.263 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.05 0.083 0.107 0.135 0.232 0.505 1.079 2.263 O.D. 0.05 0.083 0.107 0.135 0.232 0.505 1.079 2.263

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)