Human B2M ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Beta-2 microglobulin also known as B2M is a component of MHC class I molecules, which are present on all nucleated cells (excludes red blood cells) . In humans, the beta-2-microglobulin protein is encoded by the B2M gene. The protein has a predominantly beta-pleated sheet structure that can form amyloid fibrils in some pathological conditions. The encoded antimicrobial protein displays antibacterial activity in amniotic fluid. A mutation in this gene has been shown to result in hypercatabolic hypoproteinemia.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 947 3826 6537 1088 4107 6628 Standard deviation 44.51 198.95 431.44 53.31 238.21 483.84 CV (%) 4.7% 5.2% 6.6% 4.9% 5.8% 7.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 947 3826 6537 1088 4107 6628 Standard deviation 44.51 198.95 431.44 53.31 238.21 483.84 CV (%) 4.7% 5.2% 6.6% 4.9% 5.8% 7.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 947 3826 6537 1088 4107 6628 Mean (pg/ml) 947 3826 6537 1088 4107 6628 Standard deviation 44.51 198.95 431.44 53.31 238.21 483.84 Standard deviation 44.51 198.95 431.44 53.31 238.21 483.84 CV (%) 4.7% 5.2% 6.6% 4.9% 5.8% 7.3% CV (%) 4.7% 5.2% 6.6% 4.9% 5.8% 7.3%

Component of the class I major histocompatibility complex (MHC) . Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553) .

Catalog number Description Quantity Storage of opened/reconstituted material EZ1691-CA Rabbit anti- human B2M polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1691-DA Biotinylated goat anti- human B2M polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1691-ST Lyophilized recombinant human B2M standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1691-CA Rabbit anti- human B2M polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1691-DA Biotinylated goat anti- human B2M polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1691-ST Lyophilized recombinant human B2M standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1691-CA Rabbit anti- human B2M polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1691-CA Rabbit anti- human B2M polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1691-DA Biotinylated goat anti- human B2M polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) EZ1691-DA Biotinylated goat anti- human B2M polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1691-ST Lyophilized recombinant human B2M standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1691-ST Lyophilized recombinant human B2M standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.113 0.184 0.225 0.523 0.766 1.328 2.253 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.113 0.184 0.225 0.523 0.766 1.328 2.253 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.049 0.113 0.184 0.225 0.523 0.766 1.328 2.253 O.D. 0.049 0.113 0.184 0.225 0.523 0.766 1.328 2.253

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)