Human KLK1 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Kallikrein-1, also known as tissue kallikrein, is a protein that in humans is encoded by the KLK1 gene. This serine protease generates Lys-bradykinin by specific proteolysis of kininogen-1. KLK1 is a member of the peptidase S1 family. Its gene is mapped to 19q13.3. In all, it has got 262-amino acids which contain a putative signal peptide, followed by a short activating peptide and the protease domain. The protein is mainly found in kidney, pancreas, and salivary gland, showing a unique pattern of tissue-specific expression relative to other members of the family. KLK1 is implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

Angiogenesis, Cancer, Cardiovascular, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Proteolysis/Ubiquitin, Proteolytic Enzymes, Serum Proteins, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1468 3882 6352 1625 4190 6526 Standard deviation 67.53 260.1 349.4 95.9 301.7 417.7 CV (%) 4.6% 6.7% 5.5% 5.9% 7.2% 6.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1468 3882 6352 1625 4190 6526 Standard deviation 67.53 260.1 349.4 95.9 301.7 417.7 CV (%) 4.6% 6.7% 5.5% 5.9% 7.2% 6.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1468 3882 6352 1625 4190 6526 Mean (pg/ml) 1468 3882 6352 1625 4190 6526 Standard deviation 67.53 260.1 349.4 95.9 301.7 417.7 Standard deviation 67.53 260.1 349.4 95.9 301.7 417.7 CV (%) 4.6% 6.7% 5.5% 5.9% 7.2% 6.4% CV (%) 4.6% 6.7% 5.5% 5.9% 7.2% 6.4%

Catalog number Description Quantity Storage of opened/reconstituted material EZ0903-CA Rabbit anti- human KLK1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0903-DA Biotinylated goat anti- human KLK1 polyclonal antibody (Detection Antibody) 500 μl, 1 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0903-ST Lyophilized recombinant human KLK1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0903-CA Rabbit anti- human KLK1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0903-DA Biotinylated goat anti- human KLK1 polyclonal antibody (Detection Antibody) 500 μl, 1 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0903-ST Lyophilized recombinant human KLK1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0903-CA Rabbit anti- human KLK1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0903-CA Rabbit anti- human KLK1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0903-DA Biotinylated goat anti- human KLK1 polyclonal antibody (Detection Antibody) 500 μl, 1 μg/mL (recommended dilution 1:100) EZ0903-DA Biotinylated goat anti- human KLK1 polyclonal antibody (Detection Antibody) 500 μl, 1 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0903-ST Lyophilized recombinant human KLK1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0903-ST Lyophilized recombinant human KLK1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.084 0.157 0.322 0.513 0.965 1.516 2.02 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.084 0.157 0.322 0.513 0.965 1.516 2.02 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.016 0.084 0.157 0.322 0.513 0.965 1.516 2.02 O.D. 0.016 0.084 0.157 0.322 0.513 0.965 1.516 2.02

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)