Human CEA ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Carcinoembryonic antigen (CEA) is a complex immunoreactive glycoprotein with a molecular weight of 180,000 comprising 60% carbohydrate. It is found in adenocarcinomas of endodermally derived digestive system epithelia and in fetal colon.Carcinoembryonic antigen is one of the most widely used tumor markers in serum immunoassay determinations of carcinoma.

Cancer, Cell Type Markers, Human Protein, Microbiology, Oncoproteins/Suppressors, Protein, Tags & Cell Markers, Tumor Antigens, Tumor Associated, Tumor Biomarkers, Tumor Immunology, Tumor Suppressors, Tumor-Associated Antigens

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2.36 5.59 10.4 2.48 5.56 10.7 Standard deviation 0.113 0.235 0.53 0.171 0.395 0.556 CV (%) 4.8% 4.2% 5.1% 6.9% 7.1% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 2.36 5.59 10.4 2.48 5.56 10.7 Standard deviation 0.113 0.235 0.53 0.171 0.395 0.556 CV (%) 4.8% 4.2% 5.1% 6.9% 7.1% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 2.36 5.59 10.4 2.48 5.56 10.7 Mean (pg/ml) 2.36 5.59 10.4 2.48 5.56 10.7 Standard deviation 0.113 0.235 0.53 0.171 0.395 0.556 Standard deviation 0.113 0.235 0.53 0.171 0.395 0.556 CV (%) 4.8% 4.2% 5.1% 6.9% 7.1% 5.2% CV (%) 4.8% 4.2% 5.1% 6.9% 7.1% 5.2%

Catalog number Description Quantity Storage of opened/reconstituted material EZ0904-CA Rabbit anti- human CEACAM5 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0904-DA Biotinylated goat anti- human CEACAM5 polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0904-ST Lyophilized recombinant human CEACAM5 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0904-CA Rabbit anti- human CEACAM5 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0904-DA Biotinylated goat anti- human CEACAM5 polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0904-ST Lyophilized recombinant human CEACAM5 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0904-CA Rabbit anti- human CEACAM5 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0904-CA Rabbit anti- human CEACAM5 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0904-DA Biotinylated goat anti- human CEACAM5 polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) EZ0904-DA Biotinylated goat anti- human CEACAM5 polyclonal antibody (Detection Antibody) 500 μl, 4 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0904-ST Lyophilized recombinant human CEACAM5 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0904-ST Lyophilized recombinant human CEACAM5 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.011 0.056 0.111 0.210 0.381 0.713 1.296 1.967 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.011 0.056 0.111 0.210 0.381 0.713 1.296 1.967 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.011 0.056 0.111 0.210 0.381 0.713 1.296 1.967 O.D. 0.011 0.056 0.111 0.210 0.381 0.713 1.296 1.967

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)