Mouse TFF3 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.

Expressed in goblet cells of the intestines and colon (at protein level) . Expressed abundantly in goblet cells of intestine and colon, and at low levels in stomach. No expression in brain, lung, spleen, kidney, uterus, pancreas, liver, heart or thymus.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77.8 431.4 1236 81.5 449 1401.1 Standard deviation 4.279 24.5898 71.688 4.9715 30.532 98.077 CV (%) 5.5% 5.7% 5.8% 6.1% 6.8% 7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 77.8 431.4 1236 81.5 449 1401.1 Standard deviation 4.279 24.5898 71.688 4.9715 30.532 98.077 CV (%) 5.5% 5.7% 5.8% 6.1% 6.8% 7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 77.8 431.4 1236 81.5 449 1401.1 Mean (pg/ml) 77.8 431.4 1236 81.5 449 1401.1 Standard deviation 4.279 24.5898 71.688 4.9715 30.532 98.077 Standard deviation 4.279 24.5898 71.688 4.9715 30.532 98.077 CV (%) 5.5% 5.7% 5.8% 6.1% 6.8% 7% CV (%) 5.5% 5.7% 5.8% 6.1% 6.8% 7%

Catalog number Description Quantity Storage of opened/reconstituted material EZ1629-CA Rabbit anti- mouse Tff3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1629-DA Biotinylated goat anti- mouse Tff3 polyclonal antibody (Detection Antibody) 500 μl, 8 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1629-ST Lyophilized recombinant mouse Tff3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1629-CA Rabbit anti- mouse Tff3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1629-DA Biotinylated goat anti- mouse Tff3 polyclonal antibody (Detection Antibody) 500 μl, 8 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1629-ST Lyophilized recombinant mouse Tff3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1629-CA Rabbit anti- mouse Tff3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1629-CA Rabbit anti- mouse Tff3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1629-DA Biotinylated goat anti- mouse Tff3 polyclonal antibody (Detection Antibody) 500 μl, 8 μg/mL (recommended dilution 1:100) EZ1629-DA Biotinylated goat anti- mouse Tff3 polyclonal antibody (Detection Antibody) 500 μl, 8 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1629-ST Lyophilized recombinant mouse Tff3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1629-ST Lyophilized recombinant mouse Tff3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.667 2.501 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.667 2.501 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.667 2.501 O.D. 0.048 0.127 0.203 0.365 0.624 1.001 1.667 2.501

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)