Rat FABP3 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Heart-type fatty acid binding protein (hFABP), also known as mammary-derived growth inhibitor, is a protein that in humans is encoded by the FABP3 gene. The intracellular fatty acid-binding proteins (FABPs) belong to a multigene family. Fatty acid-binding protein 3 gene contains four exons and its function is to arrest growth of mammary epithelial cells. This gene is also a candidate tumor suppressor gene for human breast cancer. Cardiac-type fatty acid-binding protein (cFABP) from human heart muscle of three individuals was isolated and characterized as pI 5.3-cFABP.

Cardiovascular, Fatty Acid Oxidation, Fatty Acids, Lipid and Lipoprotein Metabolism, Lipids/Lipoproteins, Mesenchymal Stem Cells, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Redox Metabolism, Stem Cells

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 986 4588 267 1042 4602 Standard deviation 9.52 48.31 261.52 11.21 53.14 289.93 CV (%) 3.9% 4.9% 5.7% 4.2% 5.1% 6.3% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 244 986 4588 267 1042 4602 Standard deviation 9.52 48.31 261.52 11.21 53.14 289.93 CV (%) 3.9% 4.9% 5.7% 4.2% 5.1% 6.3% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 244 986 4588 267 1042 4602 Mean (pg/ml) 244 986 4588 267 1042 4602 Standard deviation 9.52 48.31 261.52 11.21 53.14 289.93 Standard deviation 9.52 48.31 261.52 11.21 53.14 289.93 CV (%) 3.9% 4.9% 5.7% 4.2% 5.1% 6.3% CV (%) 3.9% 4.9% 5.7% 4.2% 5.1% 6.3%

Catalog number Description Quantity Storage of opened/reconstituted material EZ1623-CA Rabbit anti- rat Fabp3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1623-DA Biotinylated goat anti- rat Fabp3 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1623-ST Lyophilized recombinant rat Fabp3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1623-CA Rabbit anti- rat Fabp3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1623-DA Biotinylated goat anti- rat Fabp3 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1623-ST Lyophilized recombinant rat Fabp3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1623-CA Rabbit anti- rat Fabp3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1623-CA Rabbit anti- rat Fabp3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1623-DA Biotinylated goat anti- rat Fabp3 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) EZ1623-DA Biotinylated goat anti- rat Fabp3 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1623-ST Lyophilized recombinant rat Fabp3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1623-ST Lyophilized recombinant rat Fabp3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411 O.D. 0.075 0.166 0.229 0.356 0.588 1.016 1.722 2.411

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)