Mouse MBL2 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

MBL2, also called mannose-binding lectin (protein C) 2, soluble or Mannose-binding lectin (MBL) is a lectin that is instrumental in innate immunity. MBL2 is mapped to chromosome 10q11.2-q21. It belongs to the class of collectins in the C-type lectin superfamily, whose function appears to be pattern recognition in the first line of defense in the pre-immune host. MBL2 recognizes carbohydrate patterns, found on the surface of a large number of pathogenic micro-organisms, including bacteria, viruses, protozoa and fungi. Binding MBL2 to a micro-organism results in activation of the lectin pathway of the complement system. Another important function of MBL2 is that this molecule binds senescent and apoptotic cells and enhances engulfment of whole, intact apoptotic cells, as well as cell debris by phagocytes.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3.2 8.4 12.8 3.6 8.9 13.1 Standard deviation 0.157 0.378 0.474 0.23 0.516 0.707 CV (%) 4.9% 4.5% 3.7% 6.4% 5.8% 5.4% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 3.2 8.4 12.8 3.6 8.9 13.1 Standard deviation 0.157 0.378 0.474 0.23 0.516 0.707 CV (%) 4.9% 4.5% 3.7% 6.4% 5.8% 5.4% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 3.2 8.4 12.8 3.6 8.9 13.1 Mean (pg/ml) 3.2 8.4 12.8 3.6 8.9 13.1 Standard deviation 0.157 0.378 0.474 0.23 0.516 0.707 Standard deviation 0.157 0.378 0.474 0.23 0.516 0.707 CV (%) 4.9% 4.5% 3.7% 6.4% 5.8% 5.4% CV (%) 4.9% 4.5% 3.7% 6.4% 5.8% 5.4%

Calcium-dependent lectin involved in innate immune defense. Binds mannose, fucose and N-acetylglucosamine on different microorganisms and activates the lectin complement pathway. Binds to late apoptotic cells, as well as to apoptotic blebs and to necrotic cells, but not to early apoptotic cells, facilitating their uptake by macrophages (By similarity) . .

Catalog number Description Quantity Storage of opened/reconstituted material EZ0806-CA Rabbit anti- mouse Mbl2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0806-DA Biotinylated goat anti- mouse Mbl2 polyclonal antibody (Detection Antibody) 500 μl, 1.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0806-ST Lyophilized recombinant mouse Mbl2 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0806-CA Rabbit anti- mouse Mbl2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0806-DA Biotinylated goat anti- mouse Mbl2 polyclonal antibody (Detection Antibody) 500 μl, 1.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0806-ST Lyophilized recombinant mouse Mbl2 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0806-CA Rabbit anti- mouse Mbl2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0806-CA Rabbit anti- mouse Mbl2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0806-DA Biotinylated goat anti- mouse Mbl2 polyclonal antibody (Detection Antibody) 500 μl, 1.5 μg/mL (recommended dilution 1:100) EZ0806-DA Biotinylated goat anti- mouse Mbl2 polyclonal antibody (Detection Antibody) 500 μl, 1.5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0806-ST Lyophilized recombinant mouse Mbl2 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0806-ST Lyophilized recombinant mouse Mbl2 standard 20 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.107 0.168 0.231 0.353 0.622 1.058 1.865 2.705 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.107 0.168 0.231 0.353 0.622 1.058 1.865 2.705 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 Concentration (pg/ml) 0 312 625 1250 2500 5000 10000 20000 O.D. 0.107 0.168 0.231 0.353 0.622 1.058 1.865 2.705 O.D. 0.107 0.168 0.231 0.353 0.622 1.058 1.865 2.705

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)