Human CD32/FCGR2b/c ELISA Kit EZ-SetTM (DIY Antibody Pairs)

CD32 is a surface receptor protein and part of a large population of B cell co-receptors, which act to modulate signaling. It has a low-affinity for IgG antibodies and down-regulates antibody production in the presence of IgG. This feedback loops essentially lowers the production of IgG produced by B cells when there is a surplus in the body.

Low affinity immunoglobulin gamma Fc region receptor II-b; IgG Fc receptor II-b; CDw32; Fc-gamma RII-b; Fc-gamma-RIIb; FcRII-b; CD32; FCGR2B; CD32, FCG2, IGFR2

Is the most broadly distributed Fc-gamma- receptor. Expressed in monocyte, neutrophils, macrophages, basophils, eosinophils, Langerhans cells, B-cells, platelets cells and placenta (endothelial cells) . Not detected in natural killer cells.

Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunoglobulins, Immunology, Innate Immunity, Lymphoid, Macrophage/Inflammation, Myeloid, Receptors, Stem Cells, T Lymphocytic Lineage

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 890 1865 168 904 2162 Standard deviation 6.62 32.93 80.2 9.75 41.6 112.42 CV (%) 4.5% 3.7% 4.3% 5.8% 4.6% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 147 890 1865 168 904 2162 Standard deviation 6.62 32.93 80.2 9.75 41.6 112.42 CV (%) 4.5% 3.7% 4.3% 5.8% 4.6% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 147 890 1865 168 904 2162 Mean (pg/ml) 147 890 1865 168 904 2162 Standard deviation 6.62 32.93 80.2 9.75 41.6 112.42 Standard deviation 6.62 32.93 80.2 9.75 41.6 112.42 CV (%) 4.5% 3.7% 4.3% 5.8% 4.6% 5.2% CV (%) 4.5% 3.7% 4.3% 5.8% 4.6% 5.2%

Receptor for the Fc region of complexed or aggregated immunoglobulins gamma. Low affinity receptor. Involved in a variety of effector and regulatory functions such as phagocytosis of immune complexes and modulation of antibody production by B- cells. Binding to this receptor results in down-modulation of previous state of cell activation triggered via antigen receptors on B-cells (BCR), T-cells (TCR) or via another Fc receptor. Isoform IIB1 fails to mediate endocytosis or phagocytosis. Isoform IIB2 does not trigger phagocytosis.

Catalog number Description Quantity Storage of opened/reconstituted material EZ1312-CA Rabbit anti- human FCGR2B/C polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1312-DA Biotinylated goat anti- human FCGR2B/C polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1312-ST Lyophilized recombinant human FCGR2B/C standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1312-CA Rabbit anti- human FCGR2B/C polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1312-DA Biotinylated goat anti- human FCGR2B/C polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1312-ST Lyophilized recombinant human FCGR2B/C standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1312-CA Rabbit anti- human FCGR2B/C polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1312-CA Rabbit anti- human FCGR2B/C polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1312-DA Biotinylated goat anti- human FCGR2B/C polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) EZ1312-DA Biotinylated goat anti- human FCGR2B/C polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1312-ST Lyophilized recombinant human FCGR2B/C standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1312-ST Lyophilized recombinant human FCGR2B/C standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.124 0.199 0.255 0.541 0.813 1.408 2.266 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.124 0.199 0.255 0.541 0.813 1.408 2.266 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 Concentration (pg/ml) 0 46.9 93.8 187.5 375 750 1500 3000 O.D. 0.007 0.124 0.199 0.255 0.541 0.813 1.408 2.266 O.D. 0.007 0.124 0.199 0.255 0.541 0.813 1.408 2.266

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)