Mouse CD23/FCER2 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 485 1882 8430 597 2347 9105 Standard deviation 24.25 105.4 522.66 42.39 185.41 783.03 CV (%) 5% 5.6% 6.2% 7.1% 7.9% 8.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 485 1882 8430 597 2347 9105 Standard deviation 24.25 105.4 522.66 42.39 185.41 783.03 CV (%) 5% 5.6% 6.2% 7.1% 7.9% 8.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 485 1882 8430 597 2347 9105 Mean (pg/ml) 485 1882 8430 597 2347 9105 Standard deviation 24.25 105.4 522.66 42.39 185.41 783.03 Standard deviation 24.25 105.4 522.66 42.39 185.41 783.03 CV (%) 5% 5.6% 6.2% 7.1% 7.9% 8.6% CV (%) 5% 5.6% 6.2% 7.1% 7.9% 8.6%

Low-affinity receptor for immunoglobulin E (IgE) and CR2/CD21. Has essential roles in the regulation of IgE production and in the differentiation of B-cells (it is a B-cell-specific antigen) .

Catalog number Description Quantity Storage of opened/reconstituted material EZ0942-CA Rabbit anti- mouse Fcer2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0942-DA Biotinylated goat anti- mouse Fcer2 polyclonal antibody (Detection Antibody) 500 μl, 13 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0942-ST Lyophilized recombinant mouse Fcer2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0942-CA Rabbit anti- mouse Fcer2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0942-DA Biotinylated goat anti- mouse Fcer2 polyclonal antibody (Detection Antibody) 500 μl, 13 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0942-ST Lyophilized recombinant mouse Fcer2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0942-CA Rabbit anti- mouse Fcer2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0942-CA Rabbit anti- mouse Fcer2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0942-DA Biotinylated goat anti- mouse Fcer2 polyclonal antibody (Detection Antibody) 500 μl, 13 μg/mL (recommended dilution 1:100) EZ0942-DA Biotinylated goat anti- mouse Fcer2 polyclonal antibody (Detection Antibody) 500 μl, 13 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0942-ST Lyophilized recombinant mouse Fcer2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0942-ST Lyophilized recombinant mouse Fcer2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.045 0.137 0.279 0.537 1.009 1.503 2.141 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.045 0.137 0.279 0.537 1.009 1.503 2.141 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.021 0.045 0.137 0.279 0.537 1.009 1.503 2.141 O.D. 0.021 0.045 0.137 0.279 0.537 1.009 1.503 2.141

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)