Human XCL1/Lymphotactin ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Chemokine (C motif) ligand (XCL1) is a small cytokine belonging to the XC chemokine family that is also known as lymphotactin. This gene is mapped to chromosome 1q23 by FISH. The sequence of the deduced 114-amino acid protein is most homologous to the CC chemokines CCL8 and CCL3. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces the migration of cells expressing XCR1.

Lymphotactin; ATAC; C motif chemokine 1; Cytokine SCM-1; Lymphotaxin; SCM-1-alpha; Small-inducible cytokine C1; XC chemokine ligand 1; XCL1; LTN; SCYC1

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.08 2.12 3.85 1.42 2.56 3.78 Standard deviation 0.045 0.076 0.2 0.097 0.184 0.295 CV (%) 4.2% 3.6% 5.2% 6.8% 7.2% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.08 2.12 3.85 1.42 2.56 3.78 Standard deviation 0.045 0.076 0.2 0.097 0.184 0.295 CV (%) 4.2% 3.6% 5.2% 6.8% 7.2% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1.08 2.12 3.85 1.42 2.56 3.78 Mean (pg/ml) 1.08 2.12 3.85 1.42 2.56 3.78 Standard deviation 0.045 0.076 0.2 0.097 0.184 0.295 Standard deviation 0.045 0.076 0.2 0.097 0.184 0.295 CV (%) 4.2% 3.6% 5.2% 6.8% 7.2% 7.8% CV (%) 4.2% 3.6% 5.2% 6.8% 7.2% 7.8%

Chemotactic activity for lymphocytes but not for monocytes or neutrophils. In thymus, mediates medullary accumulation of thymic dendritic cells and contributes to regulatoy T cell development, playing a role in self-tolerance establishment.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0802-CA Rabbit anti- human XCL1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0802-DA Biotinylated goat anti- human XCL1 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0802-ST Lyophilized recombinant human XCL1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0802-CA Rabbit anti- human XCL1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0802-DA Biotinylated goat anti- human XCL1 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0802-ST Lyophilized recombinant human XCL1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0802-CA Rabbit anti- human XCL1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0802-CA Rabbit anti- human XCL1 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0802-DA Biotinylated goat anti- human XCL1 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) EZ0802-DA Biotinylated goat anti- human XCL1 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0802-ST Lyophilized recombinant human XCL1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0802-ST Lyophilized recombinant human XCL1 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.142 0.144 0.146 0.182 0.228 0.372 0.833 1.717 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.142 0.144 0.146 0.182 0.228 0.372 0.833 1.717 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 Concentration (pg/ml) 0 62.5 125 250 500 1000 2000 4000 O.D. 0.142 0.144 0.146 0.182 0.228 0.372 0.833 1.717 O.D. 0.142 0.144 0.146 0.182 0.228 0.372 0.833 1.717

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)