EZ Set Accessory Kit
The EZ Set Accessory Kit provides an easy way to get all the reagents you need for performing an ELISA assay. The components included in the EZ Set Accessory Kit can also be substituted with products other sources if it is of convenience to you.
Product Specifications
Components
PART PART # DESCRIPTION QUANTITY Microplates AR1100 96-well removable strip microplate 5 Plate Sealers EK-SEA Adhesive strips 20 Reagent Diluent (10X) AR1106 10% BSA in PBS 20 mL/vial x2 Stop Solution AR1105 2N H2SO4 10 mL/vialx5 Color Developing Reagent AR1104 TMB 10 mL/vialx5 PBS (20 X) AR2100 25 mL/vial x2 PBS-T (20 X) AR2101 2% Tween® 20 in PBS 25 mL/vial x2 PART PART # DESCRIPTION QUANTITY Microplates AR1100 96-well removable strip microplate 5 Plate Sealers EK-SEA Adhesive strips 20 Reagent Diluent (10X) AR1106 10% BSA in PBS 20 mL/vial x2 Stop Solution AR1105 2N H2SO4 10 mL/vialx5 Color Developing Reagent AR1104 TMB 10 mL/vialx5 PBS (20 X) AR2100 25 mL/vial x2 PBS-T (20 X) AR2101 2% Tween® 20 in PBS 25 mL/vial x2 PART PART # DESCRIPTION QUANTITY PART PART # DESCRIPTION QUANTITY Microplates AR1100 96-well removable strip microplate 5 Microplates AR1100 96-well removable strip microplate 5 Plate Sealers EK-SEA Adhesive strips 20 Plate Sealers EK-SEA Adhesive strips 20 Reagent Diluent (10X) AR1106 10% BSA in PBS 20 mL/vial x2 Reagent Diluent (10X) AR1106 10% BSA in PBS 20 mL/vial x2 Stop Solution AR1105 2N H2SO4 10 mL/vialx5 Stop Solution AR1105 2N H2SO4 10 mL/vialx5 Color Developing Reagent AR1104 TMB 10 mL/vialx5 Color Developing Reagent AR1104 TMB 10 mL/vialx5 PBS (20 X) AR2100 25 mL/vial x2 PBS (20 X) AR2100 25 mL/vial x2 PBS-T (20 X) AR2101 2% Tween® 20 in PBS 25 mL/vial x2 PBS-T (20 X) AR2101 2% Tween® 20 in PBS 25 mL/vial x2
Storage Conditions
Store at 4°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Material Required But Not Provided
1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)
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