Human TAFI/CPB2 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.

Amino Acid Metabolism, Amino Acids, Atherosclerosis, Cardiovascular, Heart Disease, Metabolic Signaling Pathways, Metabolism, Pathways and Processes, Serum Proteins, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 752 2639 6784 886 2947 7253 Standard deviation 38.35 139.86 434.17 57.59 221.02 594.74 CV (%) 5.1% 5.3% 6.4% 6.5% 7.5% 8.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 752 2639 6784 886 2947 7253 Standard deviation 38.35 139.86 434.17 57.59 221.02 594.74 CV (%) 5.1% 5.3% 6.4% 6.5% 7.5% 8.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 752 2639 6784 886 2947 7253 Mean (pg/ml) 752 2639 6784 886 2947 7253 Standard deviation 38.35 139.86 434.17 57.59 221.02 594.74 Standard deviation 38.35 139.86 434.17 57.59 221.02 594.74 CV (%) 5.1% 5.3% 6.4% 6.5% 7.5% 8.2% CV (%) 5.1% 5.3% 6.4% 6.5% 7.5% 8.2%

Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. .

Catalog number Description Quantity Storage of opened/reconstituted material EZ1502-CA Rabbit anti- human CPB2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1502-DA Biotinylated goat anti- human CPB2 polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1502-ST Lyophilized recombinant human CPB2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1502-CA Rabbit anti- human CPB2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1502-DA Biotinylated goat anti- human CPB2 polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1502-ST Lyophilized recombinant human CPB2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1502-CA Rabbit anti- human CPB2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1502-CA Rabbit anti- human CPB2 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1502-DA Biotinylated goat anti- human CPB2 polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) EZ1502-DA Biotinylated goat anti- human CPB2 polyclonal antibody (Detection Antibody) 500 μl, 6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1502-ST Lyophilized recombinant human CPB2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1502-ST Lyophilized recombinant human CPB2 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.137 0.227 0.314 0.583 1.091 1.693 2.429 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.137 0.227 0.314 0.583 1.091 1.693 2.429 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.056 0.137 0.227 0.314 0.583 1.091 1.693 2.429 O.D. 0.056 0.137 0.227 0.314 0.583 1.091 1.693 2.429

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)