Mouse CXCL3 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Chemokine (C-X-C motif) ligand 3 (CXCL3) is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2 in rat. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37.7 118 621 38.2 128 642 Standard deviation 1.39 5.43 32.91 2.56 8.7 49.43 CV (%) 3.7% 4.6% 5.3% 6.7% 6.8% 7.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 37.7 118 621 38.2 128 642 Standard deviation 1.39 5.43 32.91 2.56 8.7 49.43 CV (%) 3.7% 4.6% 5.3% 6.7% 6.8% 7.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 37.7 118 621 38.2 128 642 Mean (pg/ml) 37.7 118 621 38.2 128 642 Standard deviation 1.39 5.43 32.91 2.56 8.7 49.43 Standard deviation 1.39 5.43 32.91 2.56 8.7 49.43 CV (%) 3.7% 4.6% 5.3% 6.7% 6.8% 7.7% CV (%) 3.7% 4.6% 5.3% 6.7% 6.8% 7.7%

Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. .

Catalog number Description Quantity Storage of opened/reconstituted material EZ1364-CA Rabbit anti- mouse Cxcl3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1364-DA Biotinylated goat anti- mouse Cxcl3 polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1364-ST Lyophilized recombinant mouse Cxcl3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ1364-CA Rabbit anti- mouse Cxcl3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1364-DA Biotinylated goat anti- mouse Cxcl3 polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1364-ST Lyophilized recombinant mouse Cxcl3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ1364-CA Rabbit anti- mouse Cxcl3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1364-CA Rabbit anti- mouse Cxcl3 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ1364-DA Biotinylated goat anti- mouse Cxcl3 polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) EZ1364-DA Biotinylated goat anti- mouse Cxcl3 polyclonal antibody (Detection Antibody) 500 μl, 12 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK1364-ST Lyophilized recombinant mouse Cxcl3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK1364-ST Lyophilized recombinant mouse Cxcl3 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.082 0.133 0.187 0.347 0.539 1.142 1.848 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.082 0.133 0.187 0.347 0.539 1.142 1.848 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 Concentration (pg/ml) 0 15.6 31.2 62.5 125 250 500 1000 O.D. 0.041 0.082 0.133 0.187 0.347 0.539 1.142 1.848 O.D. 0.041 0.082 0.133 0.187 0.347 0.539 1.142 1.848

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)