Human ADAMTS13 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

ADAMTS13 is a zinc-containing metalloprotease enzyme. The ADAMTS13 gene is mapped to chromosome 9q34 by genomic sequence analysis. It is reported that a metal-containing proteolytic enzyme (metalloprotease) in normal plasma cleaves the peptide bond between tyrosine at position 842 and methionine at position 843 in monomeric subunits of von Willebrand factor, thereby degrading the large multimers. And it is confirmed that the ADAMTS13 gene encodes the von Willebrand factor-cleaving protease (VWFCP) . ADAMTS13 is secreted in blood and degrades large vWf multimers, decreasing their activity.

Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1528 1014 1084 1369 1249 45.45 3.6% Sample 2 3313 4214 3564 5617 4177 157.06 3.8% Sample 3 17463 18584 16884 18250 17840 736.80 4.1% Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Lots Lot 1 (pg/ml) Lot 2 (pg/ml) Lot 3 (pg/ml) Lot 4 (pg/ml) Mean (pg/ml) Standard Deviation CV (%) Sample 1 1528 1014 1084 1369 1249 45.45 3.6% Sample 1 1528 1014 1084 1369 1249 45.45 3.6% Sample 2 3313 4214 3564 5617 4177 157.06 3.8% Sample 2 3313 4214 3564 5617 4177 157.06 3.8% Sample 3 17463 18584 16884 18250 17840 736.80 4.1% Sample 3 17463 18584 16884 18250 17840 736.80 4.1%

A disintegrin and metalloproteinase with thrombospondin motifs 13; ADAM-TS 13; ADAM-TS13; ADAMTS-13;3.4.24.87; von Willebrand factor-cleaving protease; vWF-CP; vWF-cleaving protease; ADAMTS13; C9orf8; UNQ6102/PRO20085

Cancer, Cell Biology, Cytoskeleton/ECM, ECM Enzymes, Extracellular Matrix, Invasion/Microenvironment, Metalloprotease, Proteolysis/Ubiquitin, Proteolytic Enzymes, Signal Transduction

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1528 3313 17643 788 5065 19529 Standard deviation 113.07 91.77 1051.52 19.78 127.13 925.67 CV (%) 7.4% 2.8% 6.0% 2.5% 2.5% 4.7% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1528 3313 17643 788 5065 19529 Standard deviation 113.07 91.77 1051.52 19.78 127.13 925.67 CV (%) 7.4% 2.8% 6.0% 2.5% 2.5% 4.7% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1528 3313 17643 788 5065 19529 Mean (pg/ml) 1528 3313 17643 788 5065 19529 Standard deviation 113.07 91.77 1051.52 19.78 127.13 925.67 Standard deviation 113.07 91.77 1051.52 19.78 127.13 925.67 CV (%) 7.4% 2.8% 6.0% 2.5% 2.5% 4.7% CV (%) 7.4% 2.8% 6.0% 2.5% 2.5% 4.7%

Catalog number Description Quantity Storage of opened/reconstituted material EZ0927-CA Rabbit anti- human ADAMTS13 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0927-DA Biotinylated goat anti- human ADAMTS13 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0927-ST Lyophilized recombinant human ADAMTS13 standard 50 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0927-CA Rabbit anti- human ADAMTS13 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0927-DA Biotinylated goat anti- human ADAMTS13 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0927-ST Lyophilized recombinant human ADAMTS13 standard 50 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0927-CA Rabbit anti- human ADAMTS13 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0927-CA Rabbit anti- human ADAMTS13 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0927-DA Biotinylated goat anti- human ADAMTS13 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) EZ0927-DA Biotinylated goat anti- human ADAMTS13 polyclonal antibody (Detection Antibody) 500 μl, 10 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0927-ST Lyophilized recombinant human ADAMTS13 standard 50 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0927-ST Lyophilized recombinant human ADAMTS13 standard 50 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.079 0.208 0.295 0.480 0.733 1.228 1.649 2.001 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.079 0.208 0.295 0.480 0.733 1.228 1.649 2.001 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 Concentration (ng/ml) 0 0.78 1.56 3.12 6.25 12.5 25 50 O.D. 0.079 0.208 0.295 0.480 0.733 1.228 1.649 2.001 O.D. 0.079 0.208 0.295 0.480 0.733 1.228 1.649 2.001

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)