Human BMP-7 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Bone morphogenetic protein 7 or BMP7 (also known as osteogenic protein-1 or OP-1) is a protein that in humans is encoded by the BMP7 gene.The protein encoded by this gene is a member of the TGF-beta superfamily. Like other members of the bone morphogenetic protein family of proteins, it plays a key role in the transformation of mesenchymal cells into bone and cartilage. It is inhibited by noggin and a similar protein, chordin, which are expressed in the Spemann-Mangold Organizer. BMP7 may be involved in bone homeostasis. It is expressed in the brain, kidneys and bladder.

Extracellular Matrix, Growth Factors/Hormones, Lipid and Lipoprotein Metabolism, Lipid Metabolism, Metabolic Signaling Pathways, Metabolism, Nervous System Development, Organogenesis, Pathways and Processes, Secreted, Signal Transduction, Signaling Pathways, Stem Cells, Structures, TGF Beta

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 342 765 1108 372 780 1265 Standard deviation 19.8 42.8 57.6 30.5 62.4 98.7 CV (%) 5.8% 5.6% 5.2% 8.2% 8% 7.8% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 342 765 1108 372 780 1265 Standard deviation 19.8 42.8 57.6 30.5 62.4 98.7 CV (%) 5.8% 5.6% 5.2% 8.2% 8% 7.8% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 342 765 1108 372 780 1265 Mean (pg/ml) 342 765 1108 372 780 1265 Standard deviation 19.8 42.8 57.6 30.5 62.4 98.7 Standard deviation 19.8 42.8 57.6 30.5 62.4 98.7 CV (%) 5.8% 5.6% 5.2% 8.2% 8% 7.8% CV (%) 5.8% 5.6% 5.2% 8.2% 8% 7.8%

Induces cartilage and bone formation. May be the osteoinductive factor responsible for the phenomenon of epithelial osteogenesis. Plays a role in calcium regulation and bone homeostasis.

Catalog number Description Quantity Storage of opened/reconstituted material EZ0884-CA Rabbit anti- human BMP7 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0884-DA Biotinylated goat anti- human BMP7 polyclonal antibody (Detection Antibody) 500 μl, 50 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0884-ST Lyophilized recombinant human BMP7 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0884-CA Rabbit anti- human BMP7 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0884-DA Biotinylated goat anti- human BMP7 polyclonal antibody (Detection Antibody) 500 μl, 50 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0884-ST Lyophilized recombinant human BMP7 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0884-CA Rabbit anti- human BMP7 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0884-CA Rabbit anti- human BMP7 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0884-DA Biotinylated goat anti- human BMP7 polyclonal antibody (Detection Antibody) 500 μl, 50 μg/mL (recommended dilution 1:100) EZ0884-DA Biotinylated goat anti- human BMP7 polyclonal antibody (Detection Antibody) 500 μl, 50 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0884-ST Lyophilized recombinant human BMP7 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0884-ST Lyophilized recombinant human BMP7 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 Concentration (pg/ml) 0 31.2 62.5 125 250 500 1000 2000 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576 O.D. 0.019 0.084 0.143 0.291 0.485 0.979 1.746 2.576

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)