Human CXCL16 ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells. CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.

C-X-C motif chemokine 16; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SR-PSOX; Small-inducible cytokine B16; Transmembrane chemokine CXCL16; CXCL16; SCYB16, SRPSOX; UNQ2759/PRO6714

Expressed in T-cell areas. Expressed in spleen, lymph nodes, lung, kidney, small intestine and thymus. Weak expression in heart and liver and no expression in brain and bone marrow.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.8 2.7 4.1 1.9 2.7 3.9 Standard deviation 0.085 0.17 0.14 0.12 0.21 0.22 CV (%) 4.7% 6.2% 3.3% 6.1% 7.8% 5.6% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1.8 2.7 4.1 1.9 2.7 3.9 Standard deviation 0.085 0.17 0.14 0.12 0.21 0.22 CV (%) 4.7% 6.2% 3.3% 6.1% 7.8% 5.6% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1.8 2.7 4.1 1.9 2.7 3.9 Mean (pg/ml) 1.8 2.7 4.1 1.9 2.7 3.9 Standard deviation 0.085 0.17 0.14 0.12 0.21 0.22 Standard deviation 0.085 0.17 0.14 0.12 0.21 0.22 CV (%) 4.7% 6.2% 3.3% 6.1% 7.8% 5.6% CV (%) 4.7% 6.2% 3.3% 6.1% 7.8% 5.6%

Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity) . Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. .

Catalog number Description Quantity Storage of opened/reconstituted material EZ0741-CA Rabbit anti- human CXCL16 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0741-DA Biotinylated goat anti- human CXCL16 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0741-ST Lyophilized recombinant human CXCL16 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0741-CA Rabbit anti- human CXCL16 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0741-DA Biotinylated goat anti- human CXCL16 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0741-ST Lyophilized recombinant human CXCL16 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0741-CA Rabbit anti- human CXCL16 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0741-CA Rabbit anti- human CXCL16 polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0741-DA Biotinylated goat anti- human CXCL16 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) EZ0741-DA Biotinylated goat anti- human CXCL16 polyclonal antibody (Detection Antibody) 500 μl, 5 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0741-ST Lyophilized recombinant human CXCL16 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0741-ST Lyophilized recombinant human CXCL16 standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.055 0.204 0.358 0.787 0.906 1.686 2.029 2.12 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.055 0.204 0.358 0.787 0.906 1.686 2.029 2.12 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 Concentration (pg/ml) 0 93.7 187.5 375 750 1500 3000 6000 O.D. 0.055 0.204 0.358 0.787 0.906 1.686 2.029 2.12 O.D. 0.055 0.204 0.358 0.787 0.906 1.686 2.029 2.12

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)