Mouse Prolactin ELISA Kit EZ-SetTM (DIY Antibody Pairs)

Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.

Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1490 2929 4543 1272 2683 4726 Standard deviation 67.05 158.2 177.2 99.22 153 246 CV (%) 4.5% 5.4% 3.9% 7.8% 5.7% 5.2% Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 Mean (pg/ml) 1490 2929 4543 1272 2683 4726 Standard deviation 67.05 158.2 177.2 99.22 153 246 CV (%) 4.5% 5.4% 3.9% 7.8% 5.7% 5.2% Intra-Assay Precision Inter-Assay Precision Intra-Assay Precision Inter-Assay Precision Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 16 16 16 24 24 24 n 16 16 16 24 24 24 Mean (pg/ml) 1490 2929 4543 1272 2683 4726 Mean (pg/ml) 1490 2929 4543 1272 2683 4726 Standard deviation 67.05 158.2 177.2 99.22 153 246 Standard deviation 67.05 158.2 177.2 99.22 153 246 CV (%) 4.5% 5.4% 3.9% 7.8% 5.7% 5.2% CV (%) 4.5% 5.4% 3.9% 7.8% 5.7% 5.2%

Catalog number Description Quantity Storage of opened/reconstituted material EZ0594-CA Rabbit anti- mouse Prl polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0594-DA Biotinylated goat anti- mouse Prl polyclonal antibody (Detection Antibody) 500 μl, 3.6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0594-ST Lyophilized recombinant mouse Prl standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material EZ0594-CA Rabbit anti- mouse Prl polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0594-DA Biotinylated goat anti- mouse Prl polyclonal antibody (Detection Antibody) 500 μl, 3.6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0594-ST Lyophilized recombinant mouse Prl standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. Catalog number Description Quantity Storage of opened/reconstituted material Catalog number Description Quantity Storage of opened/reconstituted material EZ0594-CA Rabbit anti- mouse Prl polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0594-CA Rabbit anti- mouse Prl polyclonal antibody (Capture Antibody) 500 μl, 0.4 mg/mL (recommended dilution 1:100) Store undiluted at 4°C for 1 month or at -20°C for 3 months provided this is within the expiration date of the kit. EZ0594-DA Biotinylated goat anti- mouse Prl polyclonal antibody (Detection Antibody) 500 μl, 3.6 μg/mL (recommended dilution 1:100) EZ0594-DA Biotinylated goat anti- mouse Prl polyclonal antibody (Detection Antibody) 500 μl, 3.6 μg/mL (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) AR1103 Avidin-Biotin-Peroxidase Complex (ABC) 500 μl (recommended dilution 1:100) EK0594-ST Lyophilized recombinant mouse Prl standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit. EK0594-ST Lyophilized recombinant mouse Prl standard 10 ng/tubex3 Discard the standard stock solution after 12 hours at 4°C. May be stored at -20°C for 48 hours provided this is within the expiration date of the kit.

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.11 0.185 0.263 0.398 0.686 1.047 1.685 2.234 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.11 0.185 0.263 0.398 0.686 1.047 1.685 2.234 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 Concentration (pg/ml) 0 156 312 625 1250 2500 5000 10000 O.D. 0.11 0.185 0.263 0.398 0.686 1.047 1.685 2.234 O.D. 0.11 0.185 0.263 0.398 0.686 1.047 1.685 2.234

1. Microplate reader in standard size. 2. Automated plate washer. 3. Incubator. 4. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. 5. Clean tubes and Eppendorf tubes. 6. 96 well microplate (Cat# AR1100) 7. Plate Sealers. 8. Capture Antibody Diluent: PBS. 9. Reagent Diluent: 1% BSA in PBS, pH 7.2-7.4, 0.2 μm filtered. 10. Substrate Solution: Tetramethylbenzidine (Cat# AR1104) 11. Stop Solution: 2 N H2SO4 (Cat# AR1105) 12. Wash Buffer (PBS and PBS-T) . Recipes: PBS: 8g NaCl, 0.2g KCl, 1.15g Na2HPO4, 0.2g KH2PO4, adjust the total volume to 1 L with distilled water, pH 7.2-7.4, 0.2 μm filtered. PBS-T: 0.1% Tween® 20 in PBS, pH 7.2-7.4. *Item 6 – 12 are included in the EZ Set Accessory Kit (EZA001)