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Anti-Eph receptor B1 Rabbit Monoclonal Antibody

Boster Bio Anti-Eph receptor B1 Rabbit Monoclonal Antibody catalog # M02811-1. Tested in WB, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Ubiquitin-like modifier-activating enzyme 1;6.2.1.45 ; Protein A1S9; Ubiquitin-activating enzyme E1; UBA1; A1S9T, UBE1

Gene Name

EPHB1

Gene ID

2047

UniProt

P54762

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Eph receptor B1

Clonality

Monoclonal

Clone

Clone: 20E05

Tissue Specificity

Detected in erythrocytes (at protein level) . Ubiquitous. .

Applications

WB, IP

Field of Research

Cell Biology, Epigenetics and Nuclear Signaling, Proteasome / Ubiquitin, Proteolysis/Ubiquitin, Ubiquitin & Ubiquitin Like Modifiers

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin- proteasome system (PubMed:1606621, PubMed:1447181) . Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181) . Essential for the formation of radiation- induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

117849 MW

Observed Molecular Weight

120 kDa

Gene Name Synonym

Ubiquitin-like modifier-activating enzyme 1

Subcellular Location

Cytoplasm . Mitochondrion . Nucleus .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

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