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Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody

Boster Bio Anti-Phospho-Histone H2A.X (S139) H2AFX Monoclonal Antibody catalog # MP00241. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Phospho Site

S139

Synonyms

Red fluorescent protein eqFP611; GFP-like chromoprotein

Gene Name

H2AFX

Gene ID

3014

UniProt

P16104

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Phospho-Histone H2A.X (S139)

Clonality

Monoclonal

Clone

Clone: AbH41

Tissue Specificity

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC) . Levels rapidly diminish as soon as ESC's differentiate (at protein levels) . Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Applications

WB, IHC, ICC, IF, IP

Field of Research

Epigenetics and Nuclear Signaling,2339, DNA/RNA, DNA Damage Response, Histones

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Pigment protein. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26053 MW

Observed Molecular Weight

15 kDa

Gene Name Synonym

Red fluorescent protein eqFP611

Subcellular Location

Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

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