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Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody

Boster Bio Anti-Phospho-Histone H3 (T3) H3F3A Monoclonal Antibody catalog # MP06819. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Specifications

Synonyms

Red fluorescent protein eqFP611; GFP-like chromoprotein

Gene Name

H3F3A

Gene ID

3020

UniProt

P84243

Host

Rabbit

Reactivity

Human

Immunogen

A synthesized peptide derived from human Phospho-Histone H3 (T3) H3 Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Clonality

Monoclonal

Clone

Clone: AEO-8

Tissue Specificity

Highly and selectively expressed by undifferentiated rather than differentiated embryonic stem cells (ESC) . Levels rapidly diminish as soon as ESC's differentiate (at protein levels) . Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinoma. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

2339, Epigenetics and Nuclear Signaling, Histones

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Pigment protein. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

26053 MW

Observed Molecular Weight

15 kDa

Gene Name Synonym

Red fluorescent protein eqFP611

Subcellular Location

Lateral cell membrane ; Single-pass type I membrane protein . Cell junction, tight junction . Colocalizes with CLDN7 at the lateral cell membrane and tight junction. .

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

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