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Anti-Cytokeratin 15 Rabbit Monoclonal Antibody

Boster Bio Anti-Cytokeratin 15 Rabbit Monoclonal Antibody catalog # M06791-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Keratin, type I cytoskeletal 15; Cytokeratin-15; CK-15; Keratin-15; K15; KRT15; KRTB

Gene Name

KRT15

Gene ID

3866

UniProt

P19012

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Cytokeratin 15

Clonality

Monoclonal

Clone

Clone: DOB-11

Tissue Specificity

Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level) . Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. .

Applications

WB, IHC, ICC, IF, Flow Cytometry

Field of Research

Class I, Cytoskeleton, Cytoskeleton/ECM, Intermediate Filaments, Signal Transduction

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Ubiquitin-like protein which can be covalently attached to target lysines as a monomer. Does not seem to be involved in protein degradation and may modulate protein subcellular localization, stability or activity. Upon oxidative stress, conjugates to various anti-oxidant enzymes, chaperones, and stress defense proteins. May also conjugate to NFKBIA, TFAP2A and FOS, negatively regulating their transcriptional activity, and to NR3C1, positively regulating its transcriptional activity. Covalent attachment to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I. .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

49212 MW

Observed Molecular Weight

49 kDa

Gene Name Synonym

Keratin, type I cytoskeletal 15

Subcellular Location

Cytoplasm .

Protein Name

T-cell surface glycoprotein CD3 zeta chain

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

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