Anti-PABPN1 Rabbit Monoclonal Antibody

Polyadenylate-binding protein 2; PABP-2; Poly (A) -binding protein 2; Nuclear poly (A) -binding protein 1; Poly (A) -binding protein II; PABII; Polyadenylate-binding nuclear protein 1; PABPN1; PAB2, PABP2

Involved in the 3'-end formation of mRNA precursors (pre-mRNA) by the addition of a poly (A) tail of 200-250 nt to the upstream cleavage product. Stimulates poly (A) polymerase (PAPOLA) conferring processivity on the poly (A) tail elongation reaction and controls also the poly (A) tail length. Increases the affinity of poly (A) polymerase for RNA. Is also present at various stages of mRNA metabolism including nucleocytoplasmic trafficking and nonsense-mediated decay (NMD) of mRNA. Cooperates with SKIP to synergistically activate E-box-mediated transcription through MYOD1 and may regulate the expression of muscle-specific genes. Binds to poly (A) and to poly (G) with high affinity. May protect the poly (A) tail from degradation (By similarity) . .

Nucleus . Cytoplasm . Nucleus speckle . Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Shuttles between the nucleus and the cytoplasm but predominantly found in the nucleus (PubMed:10688363) . Its nuclear import may involve the nucleocytoplasmic transport receptor transportin and a RAN-GTP- sensitive import mechanism (By similarity) . Is exported to the cytoplasm by a carrier-mediated pathway that is independent of mRNA traffic. Colocalizes with SKIP and poly (A) RNA in nuclear speckles (By similarity) . Intranuclear filamentous inclusions or aggregates are detected in the myocytes of patients; these inclusions contain PABPN1, ubiquitin, subunits of the proteasome and poly (A) RNA. .