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Anti-E2F1 Rabbit Monoclonal Antibody

Boster Bio Anti-E2F1 Rabbit Monoclonal Antibody catalog # M00257. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Specifications

Synonyms

Transcription factor E2F1; E2F-1; PBR3; Retinoblastoma-associated protein 1; RBAP-1; Retinoblastoma-binding protein 3; RBBP-3; pRB-binding protein E2F-1; E2F1; RBBP3

Gene Name

E2F1

Gene ID

1869

UniProt

Q01094

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human E2F1

Clonality

Monoclonal

Clone

Clone: IGD-5

Tissue Specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Applications

WB, IHC, ICC, IF, IP, Flow Cytometry

Field of Research

Cancer, Domain Families, Epigenetics and Nuclear Signaling, Forkhead Box, Oncoproteins/Suppressors, Transcription, Tumor Suppressors

Purification

Affinity-chromatography

Concentration

0.5mg/ml

Form

Liquid

Function

Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC- 3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F1 binds preferentially RB1 in a cell-cycle dependent manner. It can mediate both cell proliferation and TP53/p53-dependent apoptosis. Blocks adipocyte differentiation by binding to specific promoters repressing CEBPA binding to its target gene promoters (PubMed:20176812) . .

Storage Conditions

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

46920 MW

Observed Molecular Weight

34 kDa

Gene Name Synonym

Transcription factor E2F1

Subcellular Location

Nucleus.

Protein Name

P2X purinoceptor 1

Isotype

Rabbit IgG

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

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